A0A5F1S1I3 · A0A5F1S1I3_9HYPH
- ProteinPyruvate carboxylase
- Genepyc
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1153 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.
Catalytic activity
- hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H+
Cofactor
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 124 | ATP (UniProtKB | ChEBI) | |||
Binding site | 208 | ATP (UniProtKB | ChEBI) | |||
Active site | 301 | ||||
Binding site | 548 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 620 | substrate | |||
Binding site | 717 | Mn2+ (UniProtKB | ChEBI); via carbamate group | |||
Binding site | 746 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 748 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 881 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | metal ion binding | |
Molecular Function | pyruvate carboxylase activity | |
Biological Process | gluconeogenesis | |
Biological Process | pyruvate metabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePyruvate carboxylase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Rhizobiaceae > Rhizobium/Agrobacterium group > Rhizobium
Accessions
- Primary accessionA0A5F1S1I3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 717 | N6-carboxylysine | |||
Modified residue | 1118 | N6-biotinyllysine | |||
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 2-462 | Biotin carboxylation | |||
Domain | 128-326 | ATP-grasp | |||
Domain | 539-807 | Pyruvate carboxyltransferase | |||
Domain | 1077-1152 | Lipoyl-binding | |||
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,153
- Mass (Da)126,422
- Last updated2019-11-13 v1
- MD5 ChecksumFF3DBE8F80502FC370866D7965C5F51E
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
QERI01000011 EMBL· GenBank· DDBJ | TGE94909.1 EMBL· GenBank· DDBJ | Genomic DNA |