A0A5E5QXT6 · A0A5E5QXT6_PSEAI
- ProteinGTP 3',8-cyclase
- GenemoaA_2
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids331 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.
Catalytic activity
- AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H+ + L-methionine
Cofactor
Note: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.
Pathway
Cofactor biosynthesis; molybdopterin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 18 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 25 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 29 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 31 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 32 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | ||||
Sequence: C | ||||||
Binding site | 67 | GTP (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 71 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 98 | GTP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 122 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 159 | GTP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 193 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: M | ||||||
Binding site | 257 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | ||||
Sequence: C | ||||||
Binding site | 260 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | ||||
Sequence: C | ||||||
Binding site | 262-264 | GTP (UniProtKB | ChEBI) | ||||
Sequence: RVR | ||||||
Binding site | 274 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | GTP 3',8'-cyclase activity | |
Molecular Function | GTP binding | |
Molecular Function | metal ion binding | |
Molecular Function | S-adenosyl-L-methionine binding | |
Biological Process | Mo-molybdopterin cofactor biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGTP 3',8-cyclase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Pseudomonadaceae > Pseudomonas
Accessions
- Primary accessionA0A5E5QXT6
Interaction
Subunit
Monomer and homodimer.
Structure
Sequence
- Sequence statusComplete
- Length331
- Mass (Da)37,213
- Last updated2019-11-13 v1
- Checksum379A1B6922978633