A0A5E5PYK2 · A0A5E5PYK2_9GAMM
- ProteintRNA (cytidine(34)-2'-O)-methyltransferase
- GenetrmL
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids153 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.
Catalytic activity
- 5-carboxymethylaminomethyluridine34 in tRNA(Leu) + S-adenosyl-L-methionine = 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNA(Leu) + H+ + S-adenosyl-L-homocysteine
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 78 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: V | ||||||
Binding site | 100 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 119 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 127 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | RNA binding | |
Molecular Function | tRNA (5-carboxymethylaminomethyluridine(34)-2'-O)-methyltransferase activity | |
Molecular Function | tRNA (cytidine(34)-2'-O)-methyltransferase activity | |
Biological Process | wobble position cytosine ribose methylation | |
Biological Process | wobble position uridine ribose methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (cytidine(34)-2'-O)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > environmental samples
Accessions
- Primary accessionA0A5E5PYK2
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 2-139 | tRNA/rRNA methyltransferase SpoU type | ||||
Sequence: LNLVLFEPEIPNNTGSLIRLSANMGASLHLIKPFGFEMTDKRLRRAGLDYKELAQVFEYENFDHYLDKAKPERLFAVSSKVQTNYAEVEYQESDSFLFGPETRGLPQEILDRFEGITLPMQVGSRSLNLSNCVSIVAY |
Sequence similarities
Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length153
- Mass (Da)17,462
- Last updated2019-11-13 v1
- Checksum91FF901635C0E34B