A0A5E4E5B2 · A0A5E4E5B2_PRUDU

Function

function

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.

Catalytic activity

Features

Showing features for binding site.

146850100150200250300350400450
TypeIDPosition(s)Description
Binding site186S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site224-225S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site252-253S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site383S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmitochondrial matrix
Cellular Componentnucleus
Molecular FunctiontRNA (guanine(37)-N1)-methyltransferase activity
Biological ProcesstRNA N1-guanine methylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA (guanine(37)-N1)-methyltransferase
  • EC number
  • Alternative names
    • M1G-methyltransferase
    • tRNA [GM37] methyltransferase
    • tRNA methyltransferase 5 homolog

Gene names

    • ORF names
      ALMOND_2B015093

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Texas
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Rosales > Rosaceae > Amygdaloideae > Amygdaleae > Prunus

Accessions

  • Primary accession
    A0A5E4E5B2

Proteomes

Genome annotation databases

Subcellular Location

Mitochondrion matrix
Nucleus
Cytoplasm
Note: Predominantly in the mitochondria and in the nucleus.

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain96-464SAM-dependent methyltransferase TRM5/TYW2-type
Region311-364Disordered
Compositional bias312-346Polar residues

Sequence similarities

Belongs to the TRM5 / TYW2 family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    468
  • Mass (Da)
    53,060
  • Last updated
    2019-11-13 v1
  • Checksum
    BF9FD3597D797DCA
MLDESKFDVHLKLWALRIPRQLCKVATRILNGYLLDKPRIKPITEDPTCEKNRYMILSERVQDSDLSDVPEKNLDELKGLCKIEVVPYSLTLGYSYWGAGHVAHLNIHDELLPYKDVIAKVIYDKNYPRIKTIVNKVGTIENEFRVPKFEVLAGEDDMVTEVKQYGATFKLDYSLVYWNSRLEHEHVRLVSQFRAGEIICDMFAGIGPFAIPAAQKGCMVYANDLNPDSIRYLKINAETNKVDNRVRAYNIDARKFISQLMTVPNSDEKSDSDISMLKTGEKCDIQGNQEPKSENGRLGVEVKEVPDTVISNPDALQASSRNADSSVPPVKRPSDSCQSENESVDGTSAFVAGRRKGSKTKRMRGPEISNVKTWEHVDHVIMNLPASALQFLDAFRGLIQRKYWTGSLPWIHCYCFIRAIETQEYIISVAESGLNAHIKDPIFHRVRDVAPNKAMFCLSFRLPEGCLI

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias312-346Polar residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CABIKO010000002
EMBL· GenBank· DDBJ
VVA10556.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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