A0A5D8YFB0 · A0A5D8YFB0_9SPHI

  • Protein
    Glutamine-dependent NAD(+) synthetase
  • Gene
    nadE
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses L-glutamine as a nitrogen source.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (L-Gln route): step 1/1.

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site41Proton acceptor
Active site41Proton acceptor; for glutaminase activity
Active site112For glutaminase activity
Binding site118L-glutamine (UniProtKB | ChEBI)
Active site148Nucleophile; for glutaminase activity
Binding site182L-glutamine (UniProtKB | ChEBI)
Binding site295-302ATP (UniProtKB | ChEBI)
Binding site378deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site402ATP (UniProtKB | ChEBI)
Binding site407deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits
Binding site517deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular Functionglutaminase activity
Molecular FunctionNAD+ synthase (glutamine-hydrolyzing) activity
Molecular FunctionNAD+ synthase activity
Molecular Functionnitrilase activity
Biological ProcessNAD biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glutamine-dependent NAD(+) synthetase
  • EC number
  • Alternative names
    • NAD(+) synthase [glutamine-hydrolyzing]

Gene names

    • Name
      nadE
    • ORF names
      FW774_20020

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • BS3
  • Taxonomic lineage
    Bacteria > Bacteroidota > Sphingobacteriia > Sphingobacteriales > Sphingobacteriaceae > Pedobacter

Accessions

  • Primary accession
    A0A5D8YFB0

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain1-257CN hydrolase

Sequence similarities

Belongs to the NAD synthetase family.
In the C-terminal section; belongs to the NAD synthetase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    546
  • Mass (Da)
    60,564
  • Last updated
    2019-11-13 v1
  • Checksum
    4E33BB1CBE5D8788
MKIALAQLNYHIGNFEANTEKIIHAVERAKSEGADLVVFSELAVSGYPPRDFLEFTEFVGLCEESARKIAEACTGIACIVGLPTVNNNPLGKDLFNSAWFIEDTQVKAIVNKALLPNYDIFDEYRYFEPASTFNCIEFKGIKIALTICEDLWNINDNPLYISNPMDVLIAQQPDLMINIAASPFSYSHDDERVKVLSDNTSKYQLPLLYVNQVGAQTEIIFDGGSLAFNPGGMLLDELSYFKEDFRIYEFSDGQIQGLEPCEHKPHPDIEQIHGALILGIQDYFKKSGFKQAVLGLSGGIDSAVVCALAAEALGPQNVMAVLMPSKFSTGHSVQDALDLVENLGCRHEIIPIKEAADAFDNMMKPAFKGLPFNLAEENIQARCRAIVVMAMSNKFGYILLNTSNKSEAAVGYGTLYGDMAGAISVIGDVYKTQVYELARFINREREIIPVNTVVKPPSAELRPDQKDSDSLPEYDILDAILYQYIELKQSSAAIIAQGYDEALVRRILKLVNIAEFKRYQTPPILRVSPKAFGMGRRMPIVGKYLS

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
VTRT01000014
EMBL· GenBank· DDBJ
TZF80917.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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