A0A5C7EUF6 · A0A5C7EUF6_9ACTN

  • Protein
    DNA topoisomerase 1
  • Gene
    topA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

TypeIDPosition(s)Description
Site52Interaction with DNA
Site210Interaction with DNA
Site214Interaction with DNA
Active site359O-(5'-phospho-DNA)-tyrosine intermediate
Site361Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      E4J93_00145

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • BA40
  • Taxonomic lineage
    Bacteria > Actinomycetota > Coriobacteriia > Coriobacteriales > Coriobacteriaceae > Collinsella

Accessions

  • Primary accession
    A0A5C7EUF6

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain1-182Toprim
Region238-243Interaction with DNA
Region403-425Disordered
Region810-879Disordered
Compositional bias833-856Basic residues
Compositional bias857-879Basic and acidic residues

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    879
  • Mass (Da)
    94,851
  • Last updated
    2019-11-13 v1
  • Checksum
    6960B82EE8470FBB
MKLVIAEKNIAAQKIAQLLATGKPKNDKVYNTPVYRFDVDGEEWVSMGLAGHILAPDFPDEILFDKSEGWYSLTEDGEVLPAAVPDGLARPPYDTKRRPFLANGINIKGWKVESLPYLTWAPVIKNPAEKEIIRVLKNLAKKADSVVIATDFDREGELIGSDALSCVREVAPDVPVSRARYSALIKGEVTEAFNNLVELDQNLADAGESRQYIDLIWGAVLTRYLTLAKFGGFGNVRSAGRVQTPTLALVVERERERMAFVPEDYWQIRGLACASEQEFKIGHATARFTDKEAAETAYGHVAEATAGTVTDVARRSRKQQPPVPFNTTALQAAAASEGISPARTMRIAESLYMSGFISYPRVDNTVYPKSLDLEGIVNGLAANSPALAPVCKKVLAGPMQPTRGKVETTDHPPIHPTGQGDPTTLDGGQLKLYNLIARRFLATLMGPATIENTKLSIDVAGEPFTASGDVLVDAGFREAYPYGLKRDEQLPALEAGDTVDVHDIKLEAKQTEPPARYSQGKLVQEMEKRGLGTKSTRASIIERLYTVRYLKNDPIEPSQLGMAIIDALSQFAPRITTPEMTAELDADMTHVAEGADTQEHVVDHSRALLAGMLDALIEHKDDLGEAISDAVTADARVGACPKCGKDLVMKTSAKTRGSFIGCMGWPDCDVTYPVPSGVKVSPLEGEAAVCEHCGAPRIKCQPFRQKAYEQCVNPVCPTNYEPDLKVGECRVCAEAGRHGDLIAHKSEKSGKRFIRCTNYDDCGVSYPLPARGKLSATGEVCEHCGAPVVIVETARGPWRICVNMDCPGKEKKEKPARGKASAAKSTTKKAATAKKPAAKKAAAKKPAAKKPAAKKAAAKKAAAEKPAAKKAAAEKPAAE

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias833-856Basic residues
Compositional bias857-879Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
VOWY01000001
EMBL· GenBank· DDBJ
TXF38910.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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