A0A5C7EP20 · A0A5C7EP20_9ACTN

  • Protein
    Isoleucine--tRNA ligase
  • Gene
    ileS
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site574L-isoleucyl-5'-AMP (UniProtKB | ChEBI)
Binding site618ATP (UniProtKB | ChEBI)
Binding site905Zn2+ (UniProtKB | ChEBI)
Binding site908Zn2+ (UniProtKB | ChEBI)
Binding site925Zn2+ (UniProtKB | ChEBI)
Binding site928Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Functionaminoacyl-tRNA editing activity
Molecular FunctionATP binding
Molecular Functionisoleucine-tRNA ligase activity
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological Processisoleucyl-tRNA aminoacylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isoleucine--tRNA ligase
  • EC number
  • Alternative names
    • Isoleucyl-tRNA synthetase
      (IleRS
      )

Gene names

    • Name
      ileS
    • ORF names
      E4J93_04970

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • BA40
  • Taxonomic lineage
    Bacteria > Actinomycetota > Coriobacteriia > Coriobacteriales > Coriobacteriaceae > Collinsella

Accessions

  • Primary accession
    A0A5C7EP20

Proteomes

Subcellular Location

Keywords

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain29-653Aminoacyl-tRNA synthetase class Ia
Motif59-69'HIGH' region
Motif615-619'KMSKS' region
Domain699-857Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding
Domain902-930Zinc finger FPG/IleRS-type

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    933
  • Mass (Da)
    104,895
  • Last updated
    2019-11-13 v1
  • Checksum
    D69196058C256B76
MANEYKQTMNLPKTKFPMRAALAQREPERLKRWQQNHVYELMLKKNEGHEKFVLHDGPPYANGPIHLGHAMNKIAKDMINRYKAMQGYETPYVPGWDCHGQPIEHKVETQMGTEKFNQASTAEIRELCNKFAVENIDLQREGFKRLGVLGDWDNPYLTLYHQHDAADIEAFKAMFDRGMVYRGHKPVHWCKHCHTALAEAEIEYADETSPSIYVRFELTSKPAGLESFDGPVDFVIWTTTPWTIPSDQAVSLKPEAIYTAIEHDGRAEIMLKDLAEKVCGIAGWDVTPVMVDGKPYEVPAEVLDHLHYKQPVFDGVEGVALLADYVGTEDGTGIVHNSPGHGVDDYYVCMKEGMDVCMPVDDDGRFYNGDEFGAGGPFSGMDTDEANPHIIQFLRDRGMLVAEVKITHSYPHCWRCKNPVLFRATDQWFVSMDKTGLREQALDQVRNHVSWYPEHAVNRIGAMVEQRPDWCISRQRNWGVPIPSFTCADCGEKIMNDDTLDAVIALFHEKGSDAWFTDAPESYLGDACVCPKCGGHHLKADRDILDVWWDSGVSWKAVCEYRPELKYPADVYLEGSDQHRGWFQSSLLTSVGTNGIAPYKAVISQGFTLDGQGRKMSKSLGNVIDPNKICDEMGADIIRLWVASVDTSTDVACDTEILARTSDAYRRFRNTLRFLLSVLEGQFEPTTDGVAFDELMPLDKLMLARLTQVQAEVDAAYEGFEFNRAYRTLYDFVVTELSNVYLDALKDRLYCEKPGSHEMRSAQTVVAELLSMLMRDLQPILSYTVDEAMEFAPAGCRDNQDYAALLDWYKSPITLEQANEFAGVLEASLELRGVVTKAIEAARAEGTFTKSQQVRVKATAPAEAFALLTGDKAVDLAEFYIVSEVELAEGEEFTAQIEAARGEACDRCWNLRESTGVHGEHAHICDRCASALL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
VOWY01000010
EMBL· GenBank· DDBJ
TXF36741.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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