A0A5C6MP73 · A0A5C6MP73_9TELE
- ProteinPAN2-PAN3 deadenylation complex catalytic subunit PAN2
- GenePAN2
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids1199 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1.
Catalytic activity
Cofactor
Note: Binds 2 metal cations per subunit in the catalytic exonuclease domain.
Activity regulation
Positively regulated by the regulatory subunit PAN3.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 973 | a divalent metal cation (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 975 | a divalent metal cation (UniProtKB | ChEBI); catalytic | ||||
Sequence: E | ||||||
Binding site | 1082 | a divalent metal cation (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Binding site | 1134 | a divalent metal cation (UniProtKB | ChEBI); catalytic | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Cellular Component | P-body | |
Cellular Component | PAN complex | |
Molecular Function | metal ion binding | |
Molecular Function | nucleic acid binding | |
Molecular Function | poly(A)-specific ribonuclease activity | |
Biological Process | mRNA processing | |
Biological Process | nuclear-transcribed mRNA poly(A) tail shortening | |
Biological Process | positive regulation of cytoplasmic mRNA processing body assembly |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePAN2-PAN3 deadenylation complex catalytic subunit PAN2
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Neoteleostei > Acanthomorphata > Eupercaria > Tetraodontiformes > Tetradontoidea > Tetraodontidae > Takifugu
Accessions
- Primary accessionA0A5C6MP73
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Shuttles between nucleus and cytoplasm.
Keywords
- Cellular component
Interaction
Subunit
Forms a heterotrimer with an asymmetric homodimer of the regulatory subunit PAN3 to form the poly(A)-nuclease (PAN) deadenylation complex.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 511-919 | USP | ||||
Sequence: AGLEPHIPNAYCNCMIQVLYFLEPIRCLVQNHLCQKEFCLACELGFLFHMLDLSRGDPCQASNFLRAFRTIPEASALGLILADSDEQTGKAKLGRLIQSWNRFILAQLHQETQEQEGPQAYRGASSSSLGSSGESVIGKLFGCEVENSSLCRCGKETVRTSLTLLFTMHYPEHNSQDKVKEYDFAEILKRSICLEQSTQAWCENCEKYQPTVQTRNIRCLPDVLVINCEVNSAKEAEFWRVQAEYAFNKAVQKEKTEPAKPKEPSPMPTEWCLDGEEMSSMEGFTFDTRVEDLRHVWIPLSLKMSISKNQGLEINSWSEGEELSEAEEAEGASLYDLVVTVPHIQDTRTGGNLVTHIKVGETYHQRKEGVTHQQWYLFNDFLIEPIDKTEAVQFDVSWKVPAILYYAKR |
Domain
Contains a pseudo-UCH domain. This ubiquitin C-terminal hydrolase (UCH)-like or ubiquitin specific protease (USP)-like domain is predicted to be catalytically inactive because it lacks the active site catalytic triad characteristic of thiol proteases, with residues at the equivalent structural positions that are incompatible with catalysis, and it cannot bind ubiquitin. It functions as a structural scaffold for intra- and intermolecular interactions in the complex.
The linker, or PAN3 interaction domain (PID), between the WD40 repeats and the pseudo-UCH domain mediates interaction with PAN3.
Sequence similarities
Belongs to the peptidase C19 family. PAN2 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length1,199
- Mass (Da)135,121
- Last updated2019-11-13 v1
- Checksum29887F70B30E5720
Keywords
- Technical term