A0A5C6LQD7 · A0A5C6LQD7_9BACT
- ProteinCysteine desulfurase IscS
- GeneiscS
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids405 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins.
Catalytic activity
- [sulfur carrier]-H + L-cysteine = [sulfur carrier]-SH + L-alanine
Cofactor
Pathway
Cofactor biosynthesis; iron-sulfur cluster biosynthesis.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 74-75 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 154 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 182 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 202-204 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | |||
Binding site | 243 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | |||
Active site | 329 | Cysteine persulfide intermediate | |||
Binding site | 329 | [2Fe-2S] cluster (UniProtKB | ChEBI); ligand shared with IscU; via persulfide group | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 2 iron, 2 sulfur cluster binding | |
Molecular Function | cysteine desulfurase activity | |
Molecular Function | metal ion binding | |
Molecular Function | pyridoxal phosphate binding | |
Biological Process | [2Fe-2S] cluster assembly |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCysteine desulfurase IscS
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacteroidota > Chitinophagia > Chitinophagales > Chitinophagaceae > Chitinophaga
Accessions
- Primary accessionA0A5C6LQD7
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 205 | N6-(pyridoxal phosphate)lysine | |||
Interaction
Subunit
Homodimer. Forms a heterotetramer with IscU, interacts with other sulfur acceptors.
Structure
Family & Domains
Features
Showing features for domain, coiled coil.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 6-369 | Aminotransferase class V | |||
Coiled coil | 256-290 | ||||
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length405
- Mass (Da)44,038
- Last updated2019-11-13 v1
- MD5 Checksum67CDDCF43857268EE9813C7E50C0BC2C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
VOHS01000019 EMBL· GenBank· DDBJ | TWV99081.1 EMBL· GenBank· DDBJ | Genomic DNA |