A0A5C6G6M0 · A0A5C6G6M0_9HYPO

  • Protein
    Thiamine thiazole synthase
  • Gene
    THI4
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Fe cation (UniProtKB | Rhea| CHEBI:24875 )

Note: Binds 1 Fe cation per subunit.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site83substrate
Binding site104-105substrate
Binding site112substrate
Binding site177substrate
Binding site212substrate
Binding site227substrate
Binding site279substrate
Binding site289-291substrate

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentnucleus
Molecular Functioniron ion binding
Molecular Functionpentosyltransferase activity
Biological Processthiamine biosynthetic process
Biological Processthiazole biosynthetic process

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Thiamine thiazole synthase
  • Alternative names
    • Thiazole biosynthetic enzyme
      (EC:2.4.2.60
      ) . EC:2.4.2.60 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      THI4
    • ORF names
      ED733_000879

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • Cep018-CH2
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Clavicipitaceae > Metarhizium

Accessions

  • Primary accession
    A0A5C6G6M0

Proteomes

Subcellular Location

Cytoplasm
Nucleus

Keywords

PTM/Processing

Features

Showing features for modified residue.

TypeIDPosition(s)Description
Modified residue2102,3-didehydroalanine (Cys)

Post-translational modification

During the catalytic reaction, a sulfide is transferred from Cys-210 to a reaction intermediate, generating a dehydroalanine residue.

Interaction

Subunit

Homooctamer.

Family & Domains

Sequence similarities

Belongs to the THI4 family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    321
  • Mass (Da)
    34,479
  • Last updated
    2019-11-13 v1
  • Checksum
    2DD7871360409C7A
MSPPAAVSPPQHVAELATPTSKLAVKIAANNFQTIAEMMGQWDTFKFAPIRESQVSRAMTRRYFKDLDTYAESDIVIIGAGSCGLSAAYVLGKQRPDLKICIIEASVSPGGGAWLGGQLFSAMVMRKPADAFLREVGVPFEDEGNYVVVKHAALFTSTIMSKVLSLPNIKMFNATCVEDLITRPSEEGVRIAGVVTNWTLVSMHHDDQSCMDPNTINAPLVISTTGHDGPMGAFCVKRLVSMQRIEKLGGMRGLDMNSAEDAIVKNTREIVPGLIVGGMELSEVDGANRMGPTFGAMALSGLKAAEEALKVFDLRAKENAI

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
SBHS01000031
EMBL· GenBank· DDBJ
TWU72178.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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