A0A5C6G6M0 · A0A5C6G6M0_9HYPO
- ProteinThiamine thiazole synthase
- GeneTHI4
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids321 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.
Catalytic activity
- [ADP-thiazole synthase]-L-cysteine + glycine + NAD+ = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H+
Cofactor
Note: Binds 1 Fe cation per subunit.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 83 | substrate | |||
Binding site | 104-105 | substrate | |||
Binding site | 112 | substrate | |||
Binding site | 177 | substrate | |||
Binding site | 212 | substrate | |||
Binding site | 227 | substrate | |||
Binding site | 279 | substrate | |||
Binding site | 289-291 | substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Molecular Function | iron ion binding | |
Molecular Function | pentosyltransferase activity | |
Biological Process | thiamine biosynthetic process | |
Biological Process | thiazole biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameThiamine thiazole synthase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Clavicipitaceae > Metarhizium
Accessions
- Primary accessionA0A5C6G6M0
Proteomes
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Modified residue | 210 | 2,3-didehydroalanine (Cys) | |||
Post-translational modification
During the catalytic reaction, a sulfide is transferred from Cys-210 to a reaction intermediate, generating a dehydroalanine residue.
Interaction
Subunit
Homooctamer.
Structure
Sequence
- Sequence statusComplete
- Length321
- Mass (Da)34,479
- Last updated2019-11-13 v1
- Checksum2DD7871360409C7A
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
SBHS01000031 EMBL· GenBank· DDBJ | TWU72178.1 EMBL· GenBank· DDBJ | Genomic DNA |