A0A5C5UUR9 · A0A5C5UUR9_9BACT

Function

function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.
Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 2 divalent metal cations per subunit. Magnesium or manganese.
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 1 zinc ion per subunit.

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.
Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4.

Features

Showing features for binding site, site, active site.

Type
IDPosition(s)Description
Binding site29-30D-ribulose 5-phosphate (UniProtKB | ChEBI)
Binding site30Mg2+ 2 (UniProtKB | ChEBI)
Binding site30Mg2+ 1 (UniProtKB | ChEBI)
Binding site34D-ribulose 5-phosphate (UniProtKB | ChEBI)
Site127Essential for DHBP synthase activity
Binding site141-145D-ribulose 5-phosphate (UniProtKB | ChEBI)
Binding site144Mg2+ 2 (UniProtKB | ChEBI)
Binding site165D-ribulose 5-phosphate (UniProtKB | ChEBI)
Site165Essential for DHBP synthase activity
Binding site254-258GTP (UniProtKB | ChEBI)
Binding site259Zn2+ (UniProtKB | ChEBI); catalytic
Binding site270Zn2+ (UniProtKB | ChEBI); catalytic
Binding site272Zn2+ (UniProtKB | ChEBI); catalytic
Binding site275GTP (UniProtKB | ChEBI)
Binding site297-299GTP (UniProtKB | ChEBI)
Binding site319GTP (UniProtKB | ChEBI)
Active site331Proton acceptor; for GTP cyclohydrolase activity
Active site333Nucleophile; for GTP cyclohydrolase activity
Binding site354GTP (UniProtKB | ChEBI)
Binding site359GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Molecular Function3,4-dihydroxy-2-butanone-4-phosphate synthase activity
Molecular FunctionGTP binding
Molecular FunctionGTP cyclohydrolase II activity
Molecular Functionmagnesium ion binding
Molecular Functionmanganese ion binding
Molecular Functionzinc ion binding
Biological Processriboflavin biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Riboflavin biosynthesis protein RibBA

Including 2 domains:

  • Recommended name
    3,4-dihydroxy-2-butanone 4-phosphate synthase
  • EC number
  • Short names
    DHBP synthase
  • Recommended name
    GTP cyclohydrolase-2
  • EC number
  • Alternative names
    • GTP cyclohydrolase II

Gene names

    • Name
      ribBA
    • ORF names
      Enr8_45340

Organism names

  • Taxonomic identifier
  • Strain
    • Enr8
  • Taxonomic lineage
    Bacteria > Planctomycetota > Planctomycetia > Pirellulales > Pirellulaceae > Blastopirellula

Accessions

  • Primary accession
    A0A5C5UUR9

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-202DHBP synthase
Region203-402GTP cyclohydrolase II
Domain209-377GTP cyclohydrolase II

Sequence similarities

In the C-terminal section; belongs to the GTP cyclohydrolase II family.
In the N-terminal section; belongs to the DHBP synthase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    402
  • Mass (Da)
    44,168
  • Last updated
    2019-11-13 v1
  • Checksum
    95132438BAC8E1CB
MTHRFSSVQAAVDAISRGEIVIVVDAEDRENEGDFVAAAEKITPEAVNFMITHGRGMLCASILPDAGERLDLHPIVENNNAPLQTSFITPVDHRTSKTGITAKERSDTILALADANSAVDDFVRPGHVYPLLAKEGGVLRRAGHTEAAVDLARMAGLRPAGVLCEILDEAGDRADRDQLIELAKKFKLEIISIEQLIAHRRVNEKLVARAAEAELPTKYGPFQIVVYTVSYETQEPVALIFGEPDKQEKPPLVRLHSSCFTGDLISSLRCDCGDQLHMALKRISEEGHGVLVYLPQEGRGIGLTQKIRAYSLQDSGLDTVEANHALGFKADMRDYGIGIQILKDLGLRKIRLLTNNPKKTDAFIYGGFDLEVVDQVPMASPVNEFNEKYLATKRDKMGHTLP

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
SJPF01000006
EMBL· GenBank· DDBJ
TWT29878.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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