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A0A5B9NJF4 · A0A5B9NJF4_9CAUD

Function

function

Replicates the viral genomic DNA. This polymerase possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction for proofreading purpose.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Features

Showing features for binding site, site.

Type
IDPosition(s)Description
Binding site112Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site114Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site219Mg2+ 2 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site325Mg2+ 1 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site325Mg2+ 2 (UniProtKB | ChEBI); catalytic; for 3'-5' exonuclease activity
Binding site409Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site409Mg2+ 3 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site410Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site412-414substrate
Binding site480substrate
Binding site558substrate
Site619Optimization of metal coordination by the polymerase active site
Binding site621Mg2+ 3 (UniProtKB | ChEBI); catalytic; for polymerase activity
Binding site621Mg2+ 4 (UniProtKB | ChEBI); catalytic; for polymerase activity
Site704Optimization of metal coordination by the polymerase active site
Site712Essential for viral replication

GO annotations

AspectTerm
Molecular Function3'-5' exonuclease activity
Molecular FunctionDNA binding
Molecular FunctionDNA-directed DNA polymerase activity
Molecular Functionmetal ion binding
Molecular Functionnucleotide binding
Biological Processbidirectional double-stranded viral DNA replication
Biological ProcessDNA-templated DNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA-directed DNA polymerase
  • EC number

Gene names

    • ORF names
      POTTS_92

Organism names

Accessions

  • Primary accession
    A0A5B9NJF4

Proteomes

Interaction

Subunit

Part of the replicase complex that includes the DNA polymerase, the polymerase clamp, the clamp loader complex, the single-stranded DNA binding protein, and the primase/helicase. Interacts with the polymerase clamp; this interaction constitutes the polymerase holoenzyme.

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain184-291DNA-directed DNA polymerase family B exonuclease
Domain366-493DNA-directed DNA polymerase family B multifunctional
Region378-901Polymerase
Domain553-627DNA-directed DNA polymerase family B multifunctional
Region703-706Binding of DNA in B-conformation
Region895-901Interaction with the polymerase clamp

Domain

The N-terminus contains the 3'-5' exonuclease activity. The C-terminus contains the polymerase activity and is involved in binding to the polymerase clamp protein. A beta hairpin structure is necessary for the proofreading function of the polymerase.

Sequence similarities

Belongs to the DNA polymerase type-B family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    901
  • Mass (Da)
    104,231
  • Last updated
    2019-11-13 v1
  • MD5 Checksum
    454125071E1EF597447744200FD81084
MKEFFLTIEQLGNTLHERYIDSNGVEQTRQIDYKPTLFYHSDTESKYKDIYGMNCKKKHFLDMKEARNWIRRMEDIGQDAMGMDDFKLAYLSDRYPGEIHFDPTLVRQCNYDIEVTAPEFPKPNEAKYPIDALTHYDSIADKFYVFDLLNSPYGKVTKWDPVLAGKSEAEGGDEIPQDILDRVVYLPFDNEQELLLEYLNLWEQQTPVILTGWNVEGFDNPYVYNRLKNVFGEKTARRLSPLRKVNSKIIADNFGDEREIITPMGISVLDYLDLYKKFSFTNQPSYRLDYVAEYELGVGKLEYDGPINKLRETNHQRYISYNIIDVARVQQIDKKRQFLNLSLDMAYYAKMQMQSVFSPIKTWDAIIFNSLKNDNKVIPQKKHHIPQSYPGAFVKEPTPNSYKYVVSADLTSLYPSIIRQVNISPETLHGQFAVRPLHEYIAGTAPKPSEEYSCSPNGWMYRKDIDGVVPVEIKKVFDQRKLHKGFMLAAQRNAELIKEALHNPKSSDKPEPTLDYRFDMEESIKAQLHDLNEAILMSMLDKATRTEIAGMTAQICRKLLINSLYGALGNVHFRYYDLRNATAITLFGQLALQWIERRVNEFYNETLKTDNHKYVIYGDTDSIYMCVDPLIEKIGEDKFRDTNHLVDFLDKFAKEKLEPAIGRAFQDMCDYMNNKEQLMFMDREAIACPPLGSKGLGGFWTGKKRYALNVYDMEGTRYADPKMKIMGLETQKSSTPKACQKALKECIRRMLQEGESSLQDYYKEFEQEFRKQNYMTIAGVSSANNIAQYDDNGYPGYKCPFHIRGILAYNRATKNIPDAEAIVEGSKVMVLPLRDQNPFGDKCIAWPSGTQIDPSIREDVLKYLDYSTLFQKTFVKPLSGFTSAARINYEHVATLDDILGW

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MN013081
EMBL· GenBank· DDBJ
QEG12702.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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