A0A5B8YJX2 · A0A5B8YJX2_9FLAO

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

1846100200300400500600700800
Type
IDPosition(s)Description
Site33Interaction with DNA
Site139Interaction with DNA
Site140Interaction with DNA
Site143Interaction with DNA
Site148Interaction with DNA
Site155Interaction with DNA
Active site284O-(5'-phospho-DNA)-tyrosine intermediate
Site286Interaction with DNA
Site473Interaction with DNA

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      FK178_09995

Organism names

  • Taxonomic identifier
  • Strain
    • PAMC 28998
  • Taxonomic lineage
    Bacteria > Bacteroidota > Flavobacteriia > Flavobacteriales > Flavobacteriaceae > Antarcticibacterium

Accessions

  • Primary accession
    A0A5B8YJX2

Proteomes

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain3-113Toprim
Region163-168Interaction with DNA
Region814-846Disordered
Compositional bias817-846Basic residues

Sequence similarities

Belongs to the type IA topoisomerase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    846
  • Mass (Da)
    96,182
  • Last updated
    2019-11-13 v1
  • Checksum
    F251DF4C40BA10B5
MAKNLVIVESPAKAKTIEKFLGKDYTVESSFGHIADLPTKELGVDTEGNFTPKYIVNKDKKDVVNKLKKLAKNADMVWLASDEDREGEAIAWHLAEELKLDKNKTKRIVFHEITKTAILKAIENPRNINYDLVNAQQARRVLDRLVGYELSPILWRKVKGGLSAGRVQSVSVRLIVEREREIQDFKAEASFRIDAEFTNEEGKSFKAKLPKNFDTKEEAGKFLQQNIGANFKVADLTTKPAIKSPAPPFTTSTLQQEASRKLYFSVSKTMTMAQRLYEAGHITYMRTDSVNLSDDARNGAKKEILKAYGEKYSKTRQFKGKSKGAQEAHEAIRPTHFDIHTVNADRDEVRLYELIWKRAIASQMSEAQLERTNVKIDASKHDKQFTANGEVLKFDGFLKVYMESSDEEDEEQEGMLPALRVNEKLENNYITATERFTRPPYRYTEASLVKQLEELGIGRPSTYAPTISTIQNRNYVEKGTIEGSERKYEQLILKKDKYSEKQLTETVGSDKGKLVPTAVGMVVNDFLVNHFSNILDYNFTAKVEQSFDDIAEGNEEWTTMMRDFYSGFHPQVLDVAKNAEREVGERILGEDPATGKPVSVRLGKFGPMVQIGSVEDEEKPRFAGLMPEQTLDSITYEEAMDLFKLPKEIGEYEGEPVEVNNGRFGPYVRYGKKFVSLEKGEDPMSVDMDRALELIEAKEKADAPIHMYDQKPVQKGVGRFGPFLKWNDIFINVNKKYDFDNLSKEDIEELIEDKKQKEKDKVIHHWEEEGIRVEKARWGRSHILKGKTKIELPKTVDATKLSLEEVKDILEKKAKKPAKKAAAKKPAAKKAPAKKATAKKTTSKKK

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias817-846Basic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP042476
EMBL· GenBank· DDBJ
QED38034.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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