A0A5B7TCH8 · A0A5B7TCH8_9CLOT

  • Protein
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • Gene
    ispH
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the conversion of 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate (HMBPP) into a mixture of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Acts in the terminal step of the DOXP/MEP pathway for isoprenoid precursor biosynthesis.

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 1 [4Fe-4S] cluster per subunit.

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1.
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site12[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site40(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site40dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site40isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site73(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site73dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site73isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site95[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site123(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site123dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site123isopentenyl diphosphate (UniProtKB | ChEBI)
Active site125Proton donor
Binding site162(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site190[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site218(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site218dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site218isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site219(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site219dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site219isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site220(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site220dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site220isopentenyl diphosphate (UniProtKB | ChEBI)
Binding site262(2E)-4-hydroxy-3-methylbut-2-enyl diphosphate (UniProtKB | ChEBI)
Binding site262dimethylallyl diphosphate (UniProtKB | ChEBI)
Binding site262isopentenyl diphosphate (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentribosome
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Function4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity
Molecular Functionmetal ion binding
Molecular Functionnucleic acid binding
Biological Processdimethylallyl diphosphate biosynthetic process
Biological Processisopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
Biological Processterpenoid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
  • EC number
  • Short names
    HMBPP reductase

Gene names

    • Name
      ispH
    • ORF names
      FDN13_02270

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • E03
  • Taxonomic lineage
    Bacteria > Bacillota > Clostridia > Eubacteriales > Clostridiaceae > Caloramator

Accessions

  • Primary accession
    A0A5B7TCH8

Proteomes

Subcellular Location

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain322-390S1 motif
Domain408-476S1 motif
Domain497-565S1 motif
Domain582-651S1 motif

Sequence similarities

Belongs to the IspH family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    654
  • Mass (Da)
    73,775
  • Last updated
    2019-11-13 v1
  • Checksum
    C61FEC80BF5E0D9F
MQIFIAKSAGFCFGVQNAVQKAQEVAEKGEKAATLGPIIHNRQVIEDLKSKGIDLIEDISKIEDSQKIIIRSHGISKQDYDSLIAKNAIIVDATCPYVKNIHKIVQEKYSLGYKIIIIGDREHPEIKGVNGWCDNSAIIINTVEDVNNLPKNIEKVCIVAQTTFNEEKWRNIICNLIGISKEMLIFNTICNATQIRQTEAKELSKKVDVMIVVGGKESSNTRKLYEICSENCPLTLFVESVDEIDFNSIKNASSIGITAGASTPDYVIKEVVDKLNSISKINNEQNIKVKKENKTVEKENIIANENSEMDAYFIGYNEVYEDSIVKGTVLLVNDKEVFFDIGYKSDAILPIEEASNFPVNLKEKFKVGDVYDLQVIKLNDGEGNVLVSRKALEKDEFINKLYEYKEKEDYIDVIVTKENKGGLECQYGDVKAFMPVSQVGLSKDEDIKEYLGKKLKVKIIDIKEKKNDVEIVVSRKDIIKQEKDKRVKEFFENIKEGQALKGTVKSMIESGVFVSVGDVDVFIPISEISWKRIKTPKEVLNEKDIVEFIVIKVNREDKKITGSIKRAGKEPWEEFIEKYKVDDIVDVKVLRFAEFGAFAEIIPGVDGLIHISKLSEKRINKPQEVVKIGQIVKAKIINIDTASKRVSLSLKDVE

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP040093
EMBL· GenBank· DDBJ
QCX32616.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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