A0A5B6WWL9 · A0A5B6WWL9_9ROSI
- ProteinAcireductone dioxygenase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids200 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.
Catalytic activity
- 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H+
Cofactor
Ni2+ (UniProtKB | Rhea| CHEBI:49786 )
Note: Binds either 1 Fe or Ni cation per monomer. Iron-binding promotes an acireductone dioxygenase reaction producing 2-keto-4-methylthiobutyrate, while nickel-binding promotes an acireductone dioxygenase reaction producing 3-(methylsulfanyl)propanoate.
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 99 | Fe2+ (UniProtKB | ChEBI); for iron-dependent acireductone dioxygenase activity | ||||
Sequence: H | ||||||
Binding site | 99 | Ni2+ (UniProtKB | ChEBI); for nickel-dependent acireductone dioxygenase activity | ||||
Sequence: H | ||||||
Binding site | 101 | Fe2+ (UniProtKB | ChEBI); for iron-dependent acireductone dioxygenase activity | ||||
Sequence: H | ||||||
Binding site | 101 | Ni2+ (UniProtKB | ChEBI); for nickel-dependent acireductone dioxygenase activity | ||||
Sequence: H | ||||||
Binding site | 105 | Fe2+ (UniProtKB | ChEBI); for iron-dependent acireductone dioxygenase activity | ||||
Sequence: E | ||||||
Binding site | 105 | Ni2+ (UniProtKB | ChEBI); for nickel-dependent acireductone dioxygenase activity | ||||
Sequence: E | ||||||
Binding site | 144 | Fe2+ (UniProtKB | ChEBI); for iron-dependent acireductone dioxygenase activity | ||||
Sequence: H | ||||||
Binding site | 144 | Ni2+ (UniProtKB | ChEBI); for nickel-dependent acireductone dioxygenase activity | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | acireductone dioxygenase (Ni2+-requiring) activity | |
Molecular Function | acireductone dioxygenase [iron(II)-requiring] activity | |
Molecular Function | iron ion binding | |
Molecular Function | nickel cation binding | |
Biological Process | L-methionine salvage from methylthioadenosine |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAcireductone dioxygenase
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Malvales > Malvaceae > Malvoideae > Gossypium
Accessions
- Primary accessionA0A5B6WWL9
Proteomes
Structure
Sequence
- Sequence statusComplete
- Length200
- Mass (Da)23,669
- Last updated2019-11-13 v1
- ChecksumD3B382724A7E1359
Keywords
- Technical term