A0A5B0WPY1 · A0A5B0WPY1_9GAMM
- Protein6,7-dimethyl-8-ribityllumazine synthase
- GeneribE
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids157 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.
Catalytic activity
- (2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H+
Pathway
Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 25 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | ||||
Sequence: W | ||||||
Binding site | 59-61 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | ||||
Sequence: AFE | ||||||
Binding site | 83-85 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | ||||
Sequence: AVI | ||||||
Binding site | 88-89 | (2S)-2-hydroxy-3-oxobutyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: GT | ||||||
Active site | 91 | Proton donor | ||||
Sequence: H | ||||||
Binding site | 116 | 5-amino-6-(D-ribitylamino)uracil (UniProtKB | ChEBI) | ||||
Sequence: F | ||||||
Binding site | 130 | (2S)-2-hydroxy-3-oxobutyl phosphate (UniProtKB | ChEBI) | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | riboflavin synthase complex | |
Molecular Function | 6,7-dimethyl-8-ribityllumazine synthase activity | |
Biological Process | riboflavin biosynthetic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name6,7-dimethyl-8-ribityllumazine synthase
- EC number
- Short namesDMRL synthase ; LS ; Lumazine synthase
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Cellvibrionales > Halieaceae > Pseudohalioglobus
Accessions
- Primary accessionA0A5B0WPY1
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers.
Structure
Sequence
- Sequence statusComplete
- Length157
- Mass (Da)16,604
- Last updated2019-11-13 v1
- Checksum4488D9842748386D