A0A538A7U8 · A0A538A7U8_UNCAC
- ProteinLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD
- GenegatD
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids251 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
The lipid II isoglutaminyl synthase complex catalyzes the formation of alpha-D-isoglutamine in the cell wall lipid II stem peptide. The GatD subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia. The resulting ammonia molecule is channeled to the active site of MurT.
Catalytic activity
- beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + L-glutamine + ATP + H2O = beta-D-GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala)-di-trans,octa-cis-undecaprenyl diphosphate + L-glutamate + ADP + phosphate + H+
Pathway
Cell wall biogenesis; peptidoglycan biosynthesis.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 93 | Nucleophile | |||
Binding site | 127 | substrate | |||
Active site | 194 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | carbon-nitrogen ligase activity on lipid II | |
Molecular Function | glutaminase activity | |
Molecular Function | transferase activity | |
Biological Process | cell wall organization | |
Biological Process | cobalamin biosynthetic process | |
Biological Process | glutamine metabolic process | |
Biological Process | peptidoglycan biosynthetic process | |
Biological Process | regulation of cell shape |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameLipid II isoglutaminyl synthase (glutamine-hydrolyzing) subunit GatD
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes
Accessions
- Primary accessionA0A538A7U8
Proteomes
Interaction
Subunit
Forms a heterodimer with MurT.
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 4-201 | CobB/CobQ-like glutamine amidotransferase | |||
Region | 219-251 | Disordered | |||
Compositional bias | 235-251 | Basic and acidic residues | |||
Sequence similarities
Belongs to the CobB/CobQ family. GatD subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length251
- Mass (Da)26,860
- Last updated2019-10-16 v1
- MD5 Checksum70F44EEE0E3139EDDA4E90A520B19ED7
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Compositional bias | 235-251 | Basic and acidic residues | |||
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
VAYH01000091 EMBL· GenBank· DDBJ | TMK75388.1 EMBL· GenBank· DDBJ | Genomic DNA |