A0A510I6S2 · A0A510I6S2_9VIBR

  • Protein
    Ribonuclease E
  • Gene
    rne
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

110061002003004005006007008009001,000
TypeIDPosition(s)Description
Binding site302Mg2+ (UniProtKB | ChEBI); catalytic
Binding site345Mg2+ (UniProtKB | ChEBI); catalytic
Binding site403Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site406Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      VroAM7_19820

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • AM7
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Vibrionales > Vibrionaceae > Vibrio

Accessions

  • Primary accession
    A0A510I6S2

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain39-119S1 motif
Region403-406Required for zinc-mediated homotetramerization and catalytic activity
Region509-528Disordered
Region533-564Disordered
Region580-802Disordered
Compositional bias583-636Basic and acidic residues
Compositional bias687-706Basic and acidic residues
Compositional bias749-764Basic and acidic residues
Compositional bias765-790Basic residues
Region981-1006Disordered
Compositional bias990-1006Polar residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,006
  • Mass (Da)
    112,092
  • Last updated
    2019-10-16 v1
  • Checksum
    00947D0FAAD03B76
MKRMLINATQKEELRVALVDGQRLFDLDIESPGHESKKANIYKGRITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPEGYTYQGRPSIKEVLTEGQEVIVQVEKEERGSKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDERTQLKAALSTLELPQGMGLIVRTAGVGKSAEELEWDLNVLLNHWGAIKGAADSNPAPFLIHQESNVIVRAIRDYLRRDIGEILIDSNTIYERAKEHIQLVRPDFINRVKKYDGEVPLFSHYQIESQIESAFQREVRLPSGGSIVIDPTEALTSIDINSARATKGGDIEETALNTNLEAADEIARQLRLRDLGGLVVIDFIDMTPVRHQREVENRLREAVRLDRARVQIGRISRFGLLEMSRQRLSPSLAEASHHICPRCTGTGVVRDNESLALSVLRLIEEEALKDNTAQVLAVVPVPIASYLLNEKRRSVNHIERTQEVKITVVPNSDMETPHFEVIRVREGEEFDLLSYLLPKKLEAMKEAEGKEVPETELKPKKIEEPVLKGFASSTQSAPTPAAKPAAAAAAPKKKDVEQEEKPGLFSRLFKALSSFLFGSNEEKEEPKQEETKPARDNKRNNRRDRNDRRRNPRDNRDNRDNKRRRKPRDEQATEQNETQQPNKQQNRNQKRKPKAQQADKAQEQKANAKVAEKGLQLAAEARTGSEAKPEESKAKSNAKAEKVKERRQRRKLNKSVRVKDQQASEQVAEAPVKAIETPVEKPVEAVAETQQDKDNKAESKQRRNRRSPRHLRASGQRRRRGRDRRPNPFRLRKGGVASPEMAMGKVMPRYIPKPAPKKEERVKEEAAVVATAVPAIQGGFACPEMAMGKVIIRREQPAVVETPVVEPKVQQPVETKVEAPVVEAPVVEAPVVEAPVAEPAVVEQAETTTEAAQEPVVEVVEDTVEQVAVEAPIAAPAPKAEVIKVAAKPRASAPMTKAPGPQELREITVSAAPFKAERYAPKGAGSQVATNRASSGMTKPNFN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias583-636Basic and acidic residues
Compositional bias687-706Basic and acidic residues
Compositional bias749-764Basic and acidic residues
Compositional bias765-790Basic residues
Compositional bias990-1006Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP019798
EMBL· GenBank· DDBJ
BBL89329.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

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