A0A510DUY9 · A0A510DUY9_9CREN

  • Protein
    Ketol-acid reductoisomerase (NADP(+))
  • Gene
    ilvC
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions per subunit.

Pathway

Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.
Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site28-31NADP+ (UniProtKB | ChEBI)
Binding site56NADP+ (UniProtKB | ChEBI)
Active site111
Binding site137NADP+ (UniProtKB | ChEBI)
Binding site194Mg2+ 2 (UniProtKB | ChEBI)
Binding site194Mg2+ 1 (UniProtKB | ChEBI)
Binding site198Mg2+ 1 (UniProtKB | ChEBI)
Binding site230Mg2+ 2 (UniProtKB | ChEBI)
Binding site234Mg2+ 2 (UniProtKB | ChEBI)
Binding site255substrate

GO annotations

AspectTerm
Molecular Functionketol-acid reductoisomerase activity
Molecular Functionmagnesium ion binding
Biological Processisoleucine biosynthetic process
Biological Processvaline biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ketol-acid reductoisomerase (NADP(+))
  • EC number
  • Short names
    KARI
  • Alternative names
    • Acetohydroxy-acid isomeroreductase
      (AHIR
      )
    • Alpha-keto-beta-hydroxylacyl reductoisomerase

Gene names

    • Name
      ilvC
    • ORF names
      IC006_1298
      , IC007_1273

Organism names

  • Taxonomic identifier
  • Strains
    • IC-006
    • IC-007
  • Taxonomic lineage
    Archaea > Thermoproteota > Thermoprotei > Sulfolobales > Sulfolobaceae > Sulfuracidifex

Accessions

  • Primary accession
    A0A510DUY9
  • Secondary accessions
    • A0A510E2M3

Proteomes

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain5-185KARI N-terminal Rossmann
Domain186-331KARI C-terminal knotted

Sequence similarities

Belongs to the ketol-acid reductoisomerase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    335
  • Mass (Da)
    36,889
  • Last updated
    2019-10-16 v1
  • Checksum
    E5EDD717F07E845D
MINIAKIYTDKDASLDALKGKKIAVLGYGNQGRAWALNLRDSGLDVTVGLERQGKSWEKATKDGFTPVKTDEAVRNAEVIIFLVPDMIQRTIWLEKVKPNMKKGADLVFAHGFNIHFRMIEPPADSDVYMVAPKGPGDTVRDYYVAGGGVPVLVAAYQNVSGKAMEKALAVAKGIGATRSGALESSFKEETETDLVGEQTILVGGIMELMRASFETLVEMGYQPEVAYFETINEVKMIVDIIHSKGLSGMLRAVSDTAKYGGMTVGKKVINEDVRKRIREAAERVRDGQFASEWVEEYNRGMPTVVNGLKEVDNSLEQKVANELRDLIERGKPKK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AP018929
EMBL· GenBank· DDBJ
BBG23997.1
EMBL· GenBank· DDBJ
Genomic DNA
AP018930
EMBL· GenBank· DDBJ
BBG26752.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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