A0A4Z3 · A3LT2_RAT

  • Protein
    Alpha-1,3-galactosyltransferase 2
  • Gene
    A3galt2
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    5/5

Function

function

Synthesizes the galactose-alpha(1,3)-galactose group on the glycosphingolipid isoglobotrihexosylceramide or isogloboside 3 (iGb3) by catalyzing the transfer of galactose from UDP-Galactose to its acceptor molecule Gal-beta-1,4-Glc-ceramide. Can also catalyze the addition of galactose to iGb3 itself to form polygalactose structures. Synthesis of iGb3 is the initial step in the formation of the isoglobo-series glycolipid pathway and is the precursor to isogloboside 4 (iGb4) and isoForssman glycolipids. Can glycosylate only lipids and not proteins and is solely responsible for initiating the synthesis of isoglobo-series glycosphingolipids.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Note: Binds 1 Mn2+ ion per subunit.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site107-112substrate
Binding site198Mn2+ (UniProtKB | ChEBI)
Binding site198-200substrate
Binding site200Mn2+ (UniProtKB | ChEBI)
Binding site220-223substrate
Active site290Nucleophile
Binding site332-338substrate

GO annotations

AspectTerm
Cellular ComponentGolgi apparatus
Cellular ComponentGolgi cisterna membrane
Cellular Componentvesicle
Molecular Functionalpha-1,3-galactosyltransferase activity
Molecular Functionglycosyltransferase activity
Molecular Functionmetal ion binding
Molecular FunctionN-acetyllactosaminide 3-alpha-galactosyltransferase activity
Biological Processcarbohydrate metabolic process
Biological Processcellular response to manganese ion
Biological Processglycosphingolipid biosynthetic process
Biological Processlipid glycosylation

Keywords

Enzyme and pathway databases

Protein family/group databases

    • GT6Glycosyltransferase Family 6

Chemistry

Names & Taxonomy

Protein names

  • Recommended name
    Alpha-1,3-galactosyltransferase 2
  • EC number
  • Alternative names
    • Isoglobotriaosylceramide synthase (iGb3 synthase
      ; iGb3S
      )

Gene names

    • Name
      A3galt2

Organism names

  • Taxonomic identifier
  • Strain
    • Sprague-Dawley
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    A0A4Z3
  • Secondary accessions
    • A2JQW1

Proteomes

Organism-specific databases

Subcellular Location

Golgi apparatus, Golgi stack membrane
; Single-pass type II membrane protein
Note: Also found in numerous large vesicles throughout the cytoplasm of the soma.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain1-11Cytoplasmic
Transmembrane12-31Helical; Signal-anchor for type II membrane protein
Topological domain32-339Lumenal

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis187Significant loss of activity.
Mutagenesis198-200Catalytically inactive.
Mutagenesis252Complete loss of activity.

PTM/Processing

Features

Showing features for chain, glycosylation.

TypeIDPosition(s)Description
ChainPRO_00003148721-339Alpha-1,3-galactosyltransferase 2
Glycosylation57N-linked (GlcNAc...) asparagine
Glycosylation99N-linked (GlcNAc...) asparagine

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Dorsal root ganglia neurons (at protein level). High levels in spleen, thymus and skeletal muscle, intermediate levels in lung, uterus, pituitary and heart, low levels in brain, and undetected in adrenal gland and liver.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Domain

The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.

Sequence similarities

Belongs to the glycosyltransferase 6 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    339
  • Mass (Da)
    39,548
  • Last updated
    2006-11-28 v1
  • Checksum
    171A191001F0C6CF
MALEGLRAKKRLLWRLFLSAFGLLGLYHYWFKIFRLFEVFIPMGICPMAIMPLLKDNFTGVLRHWARPEVLTCTSWGAPIIWDETFDPHVAEREARRQNLTIGLTVFAVGRYLEKYLEHFLVSAEQYFMVGQNVVYYVFTDRPEAVPHVALGQGRLLRVKPVRREKRWQDVSMARMLTLHEALGGQLGREADYVFCLDVDQYFSGNFGPEVLADLVAQLHAWHFRWPRWMLPYERDKRSAAALSLSEGDFYYHAAVFGGSVAALLKLTAHCATGQQLDREHGIEARWHDESHLNKFFWLSKPTKLLSPEFCWAEEIGWRPEIHHPRLIWAPKEYALVRT

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8L2Q356A0A8L2Q356_RATA3galt2387

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF248543
EMBL· GenBank· DDBJ
AAF82757.1
EMBL· GenBank· DDBJ
mRNA
BC061714
EMBL· GenBank· DDBJ
AAH61714.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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