A0A4Z3 · A3LT2_RAT
- ProteinAlpha-1,3-galactosyltransferase 2
- GeneA3galt2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids339 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Synthesizes the galactose-alpha(1,3)-galactose group on the glycosphingolipid isoglobotrihexosylceramide or isogloboside 3 (iGb3) by catalyzing the transfer of galactose from UDP-Galactose to its acceptor molecule Gal-beta-1,4-Glc-ceramide. Can also catalyze the addition of galactose to iGb3 itself to form polygalactose structures. Synthesis of iGb3 is the initial step in the formation of the isoglobo-series glycolipid pathway and is the precursor to isogloboside 4 (iGb4) and isoForssman glycolipids. Can glycosylate only lipids and not proteins and is solely responsible for initiating the synthesis of isoglobo-series glycosphingolipids.
Catalytic activity
- a beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + UDP-alpha-D-galactose = an alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl derivative + H+ + UDPThis reaction proceeds in the forward direction.
- a beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-Cer(d18:1(4E)) + UDP-alpha-D-galactose = an isogloboside iGb3Cer (d18:1(4E)) + H+ + UDPThis reaction proceeds in the forward direction.
- a globoside Gb3Cer + UDP-alpha-D-galactose = a globoside GalGb3Cer + H+ + UDPThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 1 Mn2+ ion per subunit.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 107-112 | substrate | ||||
Sequence: FAVGRY | ||||||
Binding site | 198 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 198-200 | substrate | ||||
Sequence: DVD | ||||||
Binding site | 200 | Mn2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 220-223 | substrate | ||||
Sequence: HAWH | ||||||
Active site | 290 | Nucleophile | ||||
Sequence: E | ||||||
Binding site | 332-338 | substrate | ||||
Sequence: KEYALVR |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | Golgi apparatus | |
Cellular Component | Golgi cisterna membrane | |
Cellular Component | vesicle | |
Molecular Function | alpha-1,3-galactosyltransferase activity | |
Molecular Function | glycosyltransferase activity | |
Molecular Function | metal ion binding | |
Molecular Function | N-acetyllactosaminide 3-alpha-galactosyltransferase activity | |
Biological Process | carbohydrate metabolic process | |
Biological Process | cellular response to manganese ion | |
Biological Process | glycosphingolipid biosynthetic process | |
Biological Process | lipid glycosylation |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Protein family/group databases
Chemistry
Names & Taxonomy
Protein names
- Recommended nameAlpha-1,3-galactosyltransferase 2
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionA0A4Z3
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Golgi apparatus, Golgi stack membrane ; Single-pass type II membrane protein
Note: Also found in numerous large vesicles throughout the cytoplasm of the soma.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-11 | Cytoplasmic | ||||
Sequence: MALEGLRAKKR | ||||||
Transmembrane | 12-31 | Helical; Signal-anchor for type II membrane protein | ||||
Sequence: LLWRLFLSAFGLLGLYHYWF | ||||||
Topological domain | 32-339 | Lumenal | ||||
Sequence: KIFRLFEVFIPMGICPMAIMPLLKDNFTGVLRHWARPEVLTCTSWGAPIIWDETFDPHVAEREARRQNLTIGLTVFAVGRYLEKYLEHFLVSAEQYFMVGQNVVYYVFTDRPEAVPHVALGQGRLLRVKPVRREKRWQDVSMARMLTLHEALGGQLGREADYVFCLDVDQYFSGNFGPEVLADLVAQLHAWHFRWPRWMLPYERDKRSAAALSLSEGDFYYHAAVFGGSVAALLKLTAHCATGQQLDREHGIEARWHDESHLNKFFWLSKPTKLLSPEFCWAEEIGWRPEIHHPRLIWAPKEYALVRT |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 187 | Significant loss of activity. | ||||
Sequence: L → P | ||||||
Mutagenesis | 198-200 | Catalytically inactive. | ||||
Sequence: DVD → AVA | ||||||
Mutagenesis | 252 | Complete loss of activity. | ||||
Sequence: Y → N |
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000314872 | 1-339 | Alpha-1,3-galactosyltransferase 2 | |||
Sequence: MALEGLRAKKRLLWRLFLSAFGLLGLYHYWFKIFRLFEVFIPMGICPMAIMPLLKDNFTGVLRHWARPEVLTCTSWGAPIIWDETFDPHVAEREARRQNLTIGLTVFAVGRYLEKYLEHFLVSAEQYFMVGQNVVYYVFTDRPEAVPHVALGQGRLLRVKPVRREKRWQDVSMARMLTLHEALGGQLGREADYVFCLDVDQYFSGNFGPEVLADLVAQLHAWHFRWPRWMLPYERDKRSAAALSLSEGDFYYHAAVFGGSVAALLKLTAHCATGQQLDREHGIEARWHDESHLNKFFWLSKPTKLLSPEFCWAEEIGWRPEIHHPRLIWAPKEYALVRT | ||||||
Glycosylation | 57 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 99 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Dorsal root ganglia neurons (at protein level). High levels in spleen, thymus and skeletal muscle, intermediate levels in lung, uterus, pituitary and heart, low levels in brain, and undetected in adrenal gland and liver.
Gene expression databases
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Domain
The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
Sequence similarities
Belongs to the glycosyltransferase 6 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length339
- Mass (Da)39,548
- Last updated2006-11-28 v1
- Checksum171A191001F0C6CF
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8L2Q356 | A0A8L2Q356_RAT | A3galt2 | 387 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF248543 EMBL· GenBank· DDBJ | AAF82757.1 EMBL· GenBank· DDBJ | mRNA | ||
BC061714 EMBL· GenBank· DDBJ | AAH61714.1 EMBL· GenBank· DDBJ | mRNA |