A0A4Y6UYX2 · A0A4Y6UYX2_SACBS

  • Protein
    Carbamoyl phosphate synthase small chain
  • Gene
    carA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site45L-glutamine (UniProtKB | ChEBI)
Binding site219L-glutamine (UniProtKB | ChEBI)
Binding site221L-glutamine (UniProtKB | ChEBI)
Active site246Nucleophile
Binding site247L-glutamine (UniProtKB | ChEBI)
Binding site250L-glutamine (UniProtKB | ChEBI)
Binding site288L-glutamine (UniProtKB | ChEBI)
Binding site291L-glutamine (UniProtKB | ChEBI)
Active site331
Active site333

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      FFV09_20035

Organism names

  • Taxonomic identifier
  • Strain
    • KCTC 43072 / ATSA2
  • Taxonomic lineage
    Bacteria > Bacillota > Bacilli > Bacillales > Paenibacillaceae > Saccharibacillus

Accessions

  • Primary accession
    A0A4Y6UYX2

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain1-131Carbamoyl-phosphate synthase small subunit N-terminal
Region1-170CPSase
Region364-416Disordered

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    416
  • Mass (Da)
    45,117
  • Last updated
    2019-09-18 v1
  • Checksum
    F5739EA2E5556592
MKARLILEDGTLFTGSSFGSDQQMTGEIVFNTGITGYQEVLSDPSYCGQIVTMTQPLIGNYGINREDFEAVRPWIHGFVVRRYENAPSNWRSDSDLGSMLKEYDIPGIADIDTRMLTRRLRNHGTLRGLITTGTASPEELLEQLQASAVTRDQVSRVSTPRPYRVPGTKQRIVVIDYGSKAGIVRELGERGCDVVVVPHAANVADIRRFNPDGVLLSNGPGDPKDVPEAIETVRQLIGQLPLFGICLGHQLFALASGADTTKLKFGHRGANHPVKDLAANRCLITSQNHSYSVLEDSLAQTDLVLTHINNNDGSVEGLKHKSAPAFSVQFHPEAAPGPTDSNLLFDQFLDMVDAFKAERAPFRSSVAQAKESATGETAGTPAPKRTAGTAAERHAKRQQADGATSPLEKVLTPSAY

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP041217
EMBL· GenBank· DDBJ
QDH22943.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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