A0A4Y5T732 · A0A4Y5T732_PRRSV
- ProteinReplicase polyprotein 1ab
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids1463 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
function
Cleaves the majority of cleavage sites present in the C-terminus of the polyprotein. Triggers host apoptosis through caspase-3, -8, and -9 activations. Subverts host innate immune responses through its protease activity. Targets the NF-kappa-B essential modulator NEMO and mediates its cleavage. Blocks host interferon beta induction and downstream signaling by cleaving mitochondrial MAVS, dislodging it from the mitochondria. Impairs host defense by cleaving host mRNA-decapping enzyme DCP1A to attenuate its antiviral activity.
Plays a role in the inhibition of the immune response by interacting with host IFITM1. This interaction leads to the proteasomal degradation of the IFN-induced antiviral protein IFITM1.
Plays a role in viral transcription/replication and prevents the simultaneous activation of host cell dsRNA sensors, such as MDA5/IFIH1, OAS, PKR (By similarity) and NLRP3 inflammasome (By similarity).
Acts by degrading the 5'-polyuridines generated during replication of the poly(A) region of viral genomic and subgenomic RNAs. Catalyzes a two-step reaction in which a 2'3'-cyclic phosphate (2'3'-cP) is first generated by 2'-O transesterification, which is then hydrolyzed to a 3'-phosphate (3'-P) (By similarity).
If not degraded, poly(U) RNA would hybridize with poly(A) RNA tails and activate host dsRNA sensors (By similarity).
Also plays a role in the inhibition of host type I interferon production by recruiting host OTULIN to promote removal of linear ubiquitination targeting host NEMO
Acts by degrading the 5'-polyuridines generated during replication of the poly(A) region of viral genomic and subgenomic RNAs. Catalyzes a two-step reaction in which a 2'3'-cyclic phosphate (2'3'-cP) is first generated by 2'-O transesterification, which is then hydrolyzed to a 3'-phosphate (3'-P) (By similarity).
If not degraded, poly(U) RNA would hybridize with poly(A) RNA tails and activate host dsRNA sensors (By similarity).
Also plays a role in the inhibition of host type I interferon production by recruiting host OTULIN to promote removal of linear ubiquitination targeting host NEMO
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 1217 | |||||
Sequence: H | ||||||
Active site | 1232 | |||||
Sequence: H | ||||||
Active site | 1261 | |||||
Sequence: K |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | host cell membrane | |
Cellular Component | host cell perinuclear region of cytoplasm | |
Cellular Component | membrane | |
Molecular Function | ATP binding | |
Molecular Function | endonuclease activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA helicase activity | |
Molecular Function | RNA nuclease activity | |
Molecular Function | RNA-dependent RNA polymerase activity | |
Molecular Function | zinc ion binding | |
Biological Process | DNA-templated transcription | |
Biological Process | viral RNA genome replication | |
Biological Process | viral translational frameshifting |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameReplicase polyprotein 1ab
- Alternative names
Organism names
- Strain
- Taxonomic lineageViruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Arnidovirineae > Arteriviridae > Variarterivirinae > Betaarterivirus > Ampobartevirus > Betaarterivirus suid 2
- Virus hosts
Accessions
- Primary accessionA0A4Y5T732
Subcellular Location
UniProt Annotation
GO Annotation
Host membrane ; Multi-pass membrane protein
Membrane ; Multi-pass membrane protein
Keywords
- Cellular component
Interaction
Subunit
Interacts with host DDX18; this interaction redistributes host DDX18 to the cytoplasm.
Interacts with host DDX5.
Interacts with host IFITM1.
Interacts with host LGALS3.
Interacts with host OTULIN.
Nsp1-alpha papain-like: Interacts with host RNF31.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 1-152 | NiRAN | ||||
Sequence: TGFKLLAASGLTRCGRGGLVITETAVKIVKYHSRTFTLGPLDLKVTSEVEIKKSTEQGHAVVANLCSGVVLMRPHPPSLVDILLKPGLDTTPGIQPGHGAGNMGVDGSAWDFETAPTKVELELSKQIIQACEVRRGDAPNLQLPYKLHPVRG | ||||||
Domain | 391-525 | RdRp catalytic | ||||
Sequence: GRCLEADLASCDRSTPAIVRWFVANLLYELAGCEEYLPSYVLNCCHDLVATQDGAFTKRGGLSSGDPVTSVSNTVYSLIIYAQHMVLSALKMGHEIGLKFLEEQLKFEDLLEIQPMLVYSDDLVLYAEKPTFPNY | ||||||
Domain | 646-709 | AV ZBD | ||||
Sequence: GKKFRHCGICDAKADHASACGLDLCLFHSHFHQHCPVTLNCGHQAGSKECSQCQSPVGAGKSPL | ||||||
Domain | 759-1048 | +RNA virus helicase C-terminal | ||||
Sequence: DLADGDYQVVPLLPTCKDINMVKVACNVLLSKFIVGPPGSGKTTWLLNQVQDDDVIYTPTHQTMFDIVSALKVCRYSIPGASGLPFPPPARSGPWVRLVASGHVPGRISYLDEAGYCNHLDILRLLSKTPLVCLGDLQQLHPVGFDSHCYVFDQMPHKQLTTIYRFGPNICAAIQPCYRERLESKARNTRVVFTTRPVAFGQVLTPYHKDRTGSAITIDSSQGATFDVVTLHLPSPNSLTKSRALVAITRARHGLFIYDPHSQLQEFFNLIPERTDCNLVFSRGDELVVL | ||||||
Domain | 1087-1184 | AV-Nsp11N/CoV-Nsp15M | ||||
Sequence: EGSCMPLPQVAHNLGFYFSPDSPAFAPLPKELAPHWPVVTHQNNRAWPDRLVASMRPIDACYSKPMVGAGYVVGPSTFLGTPGVVSYYLTLYIGGEPR | ||||||
Domain | 1186-1308 | NendoU | ||||
Sequence: LPETLVSTGRIATDCREYLDAAEEEVAKELPHAFIGDVKGTTVGGCHHITSKYLPRSLPKDSVAVVGVSSPGKAAKAVCTLTDVYLPELRPYLQPETASKCWKLKLDFRDVRLMVWKGATAYF |
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusFragment
- Length1,463
- Mass (Da)161,811
- Last updated2019-09-18 v1
- Checksum57290B5DC1BCA26D
Features
Showing features for non-terminal residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Non-terminal residue | 1 | |||||
Sequence: T |
Keywords
- Coding sequence diversity