A0A4X2LWG0 · A0A4X2LWG0_VOMUR

Function

Catalytic activity

  • Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.
    EC:3.4.24.24 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Ca2+ (UniProtKB | Rhea| CHEBI:29108 )

Note: Can bind about 5 Ca2+ ions per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per subunit.

Features

Showing features for binding site, active site.

166450100150200250300350400450500550600650
TypeIDPosition(s)Description
Binding site102Zn2+ 2 (UniProtKB | ChEBI); catalytic; in inhibited form
Binding site134Ca2+ 1 (UniProtKB | ChEBI)
Binding site168Ca2+ 2 (UniProtKB | ChEBI)
Binding site178Zn2+ 1 (UniProtKB | ChEBI)
Binding site180Zn2+ 1 (UniProtKB | ChEBI)
Binding site185Ca2+ 3 (UniProtKB | ChEBI)
Binding site186Ca2+ 3 (UniProtKB | ChEBI)
Binding site190Ca2+ 3 (UniProtKB | ChEBI)
Binding site193Zn2+ 1 (UniProtKB | ChEBI)
Binding site202Ca2+ 2 (UniProtKB | ChEBI)
Binding site204Ca2+ 2 (UniProtKB | ChEBI)
Binding site206Zn2+ 1 (UniProtKB | ChEBI)
Binding site208Ca2+ 3 (UniProtKB | ChEBI)
Binding site209Ca2+ 1 (UniProtKB | ChEBI)
Binding site211Ca2+ 3 (UniProtKB | ChEBI)
Binding site211Ca2+ 1 (UniProtKB | ChEBI)
Active site231
Active site404
Binding site480Ca2+ 4 (UniProtKB | ChEBI)
Binding site482Ca2+ 5 (UniProtKB | ChEBI)
Binding site525Ca2+ 4 (UniProtKB | ChEBI)
Binding site575Ca2+ 5 (UniProtKB | ChEBI)
Binding site622Ca2+ 4 (UniProtKB | ChEBI)
Binding site624Ca2+ 5 (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentextracellular matrix
Cellular Componentextracellular space
Cellular Componentplasma membrane
Cellular Componentsarcomere
Molecular Functionmetalloendopeptidase activity
Molecular Functionzinc ion binding
Biological Processblood vessel maturation
Biological Processbone trabecula formation
Biological Processcellular response to amino acid stimulus
Biological Processcellular response to reactive oxygen species
Biological Processcellular response to UV-A
Biological Processcollagen catabolic process
Biological Processembryo implantation
Biological Processendodermal cell differentiation
Biological Processface morphogenesis
Biological Processintramembranous ossification
Biological Processpositive regulation of vascular associated smooth muscle cell proliferation
Biological Processproteolysis
Biological Processresponse to amyloid-beta
Biological Processresponse to hypoxia

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    72 kDa type IV collagenase
  • EC number
  • Alternative names
    • 72 kDa gelatinase
    • Matrix metalloproteinase-2

Gene names

    • Name
      MMP2

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Metatheria > Diprotodontia > Vombatidae > Vombatus

Accessions

  • Primary accession
    A0A4X2LWG0

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for signal, chain, disulfide bond, modified residue.

TypeIDPosition(s)Description
Signal1-29
ChainPRO_502136343730-66472 kDa type IV collagenase
Disulfide bond233↔259
Disulfide bond247↔274
Disulfide bond291↔317
Disulfide bond305↔332
Disulfide bond349↔375
Disulfide bond363↔390
Disulfide bond471↔664
Modified residue556Phosphotyrosine; by PKDCC

Keywords

Interaction

Subunit

Interacts (via the C-terminal hemopexin-like domains-containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B.

Protein-protein interaction databases

Family & Domains

Features

Showing features for motif, domain, region, compositional bias, repeat.

TypeIDPosition(s)Description
Motif100-107Cysteine switch
Domain228-276Fibronectin type-II
Domain286-334Fibronectin type-II
Domain344-392Fibronectin type-II
Region444-468Disordered
Compositional bias446-460Polar residues
Repeat470-520Hemopexin
Repeat521-567Hemopexin
Repeat569-617Hemopexin
Repeat618-664Hemopexin

Sequence similarities

Belongs to the peptidase M10A family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    664
  • Mass (Da)
    74,643
  • Last updated
    2019-09-18 v1
  • Checksum
    1D585E2C0D6CC1AB
MMTPAAPGVLGFLLPALWLHSSVFQQTWAAPSPIIKFPGDVSPKTDKELAVQYLNTFYGCPKESCNLFVLKDTLKKMQKFFGLPETGELDQSTIETMKKPRCGNPDVANYNFFPRRPKWEKNQITYRIIGYTPDLDPETVDDAFARAFKVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNDKEYTSCTDAGRSDGFLWCATSYNFDSDGKYGFCPHEALFTMGGNAEGQPCKFPFKFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGSPCVFPFTFLGNKHESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSEDPGALMAPIYTYTKNFRLSNDDIKGIRELYGSSSDTDTGTDTGPTPTPGPVTPEICKPDTNIVFDGISQIRGETFFFKDRFMWRTANTREKPTGPLLVATFWPEVPEKIDAVYEAPQEEKAVFFSGDEYWVYSASTLERGYPKKLTSLGLPPDVQHVDAAFSWSKNKKTYIFSGDKFWRYNEVKKKMDPGFPKLIADAWNGIPDNLDAVVELQGSGHSYFFKDWYYLKLENKSLKIVKVGNIKSDWLSC

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias446-460Polar residues

Keywords

Genome annotation databases

Similar Proteins

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