A0A4W3GJ87 · A0A4W3GJ87_CALMI

Function

function

Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase. It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction. Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles.

Catalytic activity

  • (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-phenylalanine = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-phenylalanine + H2O
    This reaction proceeds in the forward
    and the backward
    directions.
  • L-phenylalanine + (9Z)-octadecenoate = N-(9Z-octadecenoyl)-L-phenylalanine + H2O
    This reaction proceeds in the forward
    and the backward
    directions.
  • (9Z)-octadecenoate + glycine = N-(9Z-octadecenoyl)glycine + H2O
    This reaction proceeds in the backward direction.
  • N-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-L-phenylalanine + H2O = (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + L-phenylalanine
    This reaction proceeds in the forward direction.
  • N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycine
    This reaction proceeds in the forward
    and the backward
    directions.
  • N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-serine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-serine
    This reaction proceeds in the forward
    and the backward
    directions.
  • N-(9Z-octadecenoyl)-L-asparagine + H2O = L-asparagine + (9Z)-octadecenoate
    This reaction proceeds in the forward direction.
  • N-(9Z-octadecenoyl)-L-glutamine + H2O = L-glutamine + (9Z)-octadecenoate
    This reaction proceeds in the forward direction.
  • N-(9Z-octadecenoyl)-L-leucine + H2O = L-leucine + (9Z)-octadecenoate
    This reaction proceeds in the forward
    and the backward
    directions.
  • N-(9Z-octadecenoyl)-L-lysine + H2O = L-lysine + (9Z)-octadecenoate
    This reaction proceeds in the forward direction.
  • N-(9Z-octadecenoyl)-L-methionine + H2O = (9Z)-octadecenoate + L-methionine
    This reaction proceeds in the forward direction.
  • N-(9Z-octadecenoyl)-L-serine + H2O = L-serine + (9Z)-octadecenoate
    This reaction proceeds in the forward direction.
  • N-(9Z-octadecenoyl)-L-tryptophan + H2O = L-tryptophan + (9Z)-octadecenoate
    This reaction proceeds in the forward direction.
  • N-(9Z-octadecenoyl)-L-tyrosine + H2O = L-tyrosine + (9Z)-octadecenoate
    This reaction proceeds in the forward direction.
  • N-hexadecanoyl-L-phenylalanine + H2O = hexadecanoate + L-phenylalanine
    This reaction proceeds in the forward direction.
  • N-octadecanoyl-L-phenylalanine + H2O = octadecanoate + L-phenylalanine
    This reaction proceeds in the forward direction.
  • an N-acyl-L-amino acid + H2O = an L-alpha-amino acid + a carboxylate
    This reaction proceeds in the forward
    and the backward
    directions.
    EC:3.5.1.14 (UniProtKB | ENZYME | Rhea)
  • an N-acyl-aromatic L-alpha-amino acid + H2O = an aromatic L-alpha-amino acid + a carboxylate
    This reaction proceeds in the forward
    and the backward
    directions.
    EC:3.5.1.114 (UniProtKB | ENZYME | Rhea)

Pathway

Amino-acid metabolism.
Lipid metabolism; fatty acid metabolism.

GO annotations

AspectTerm
Molecular Functionhydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
Molecular Functionmetal ion binding
Molecular Functionpeptidase activity
Biological Processamide biosynthetic process
Biological Processamide catabolic process
Biological Processamino acid metabolic process
Biological Processcellular lipid metabolic process
Biological Processproteolysis

Keywords

Names & Taxonomy

Protein names

  • Submitted names
    • Probable carboxypeptidase PM20D1.2

Gene names

    • Name
      PM20D1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Chondrichthyes > Holocephali > Chimaeriformes > Callorhinchidae > Callorhinchus

Accessions

  • Primary accession
    A0A4W3GJ87

Proteomes

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-28
ChainPRO_502127608329-506

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain243-384Peptidase M20 dimerisation

Sequence similarities

Belongs to the peptidase M20A family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    506
  • Mass (Da)
    56,087
  • Last updated
    2019-09-18 v1
  • Checksum
    BFB042E256A8A9C5
MQVPGWLRIVFLAVSLLCCALLLRTLSAGGGRKVDRLRAASAADFTPGENERLLSNLRAAVQIPTVSLARAQPDTAAMEAFRDYLPQAFPGVFSSDLVRHEIIANYSHLFTVRGSDPGLRPYILTAHLDVVPVAEEKWDFPPFSGEESDGYLYGRGTLDDKASAVGILQALEFLLNRGYVPRRAFYVAIGHDEEVGGDNGARNIAATLESRGVKLSFLLDEGSVILDHIIPGLKKPVAVIAVTEKGQALFSLKVNTQTGHSSMPMRESSIGILASAVSRLEKKPMPRMFGLGPELAMFEQLGPEMSFPLNIIMANLWLFAPIISRTLEKNPSMNALVRTTTAVTEFHAGIKPNVLPPTANATLNFRIHPAHSVQGLIEEMRYTIDDDRVKIDLLYGFDPLPTSPHDELAFGYQIIRTTVENVFPEVFVIPGLSVANTDTRHYQHLTEGLYRFVPLWITQEDTGRIHGPNERISVKNYEEIVRFYFHLIQNSDLNLRLNVHSSGHEL

Computationally mapped potential isoform sequences

There are 3 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A4W3GKN8A0A4W3GKN8_CALMIPM20D1523
A0A4W3GJU9A0A4W3GJU9_CALMIPM20D1508
A0A4W3H0R0A0A4W3H0R0_CALMIPM20D1495

Keywords

Sequence databases

Genome annotation databases

Similar Proteins

Disclaimer

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