A0A4W3GJ87 · A0A4W3GJ87_CALMI
- ProteinProbable carboxypeptidase PM20D1.2
- GenePM20D1
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids506 (go to sequence)
- Protein existenceInferred from homology
- Annotation score5/5
Function
function
Secreted enzyme that regulates the endogenous N-fatty acyl amino acid (NAAs) tissue and circulating levels by functioning as a bidirectional NAA synthase/hydrolase. It condenses free fatty acids and free amino acids to generate NAAs and bidirectionally catalyzes the reverse hydrolysis reaction. Some of these NAAs stimulate oxidative metabolism via mitochondrial uncoupling, increasing energy expenditure in a UPC1-independent manner. Thereby, this secreted protein may indirectly regulate whole body energy expenditure. PM20D1 circulates in tight association with both low- and high-density (LDL and HDL,respectively) lipoprotein particles.
Catalytic activity
- (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-phenylalanine = N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-phenylalanine + H2OThis reaction proceeds in the forward and the backward directions.
- L-phenylalanine + (9Z)-octadecenoate = N-(9Z-octadecenoyl)-L-phenylalanine + H2OThis reaction proceeds in the forward and the backward directions.
- (9Z)-octadecenoate + glycine = N-(9Z-octadecenoyl)glycine + H2OThis reaction proceeds in the backward direction.
- N-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-L-phenylalanine + H2O = (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + L-phenylalanineThis reaction proceeds in the forward direction.
- N-(5Z,8Z,11Z,14Z)-eicosatetraenoyl-glycine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycineThis reaction proceeds in the forward and the backward directions.
- N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-L-serine + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + L-serineThis reaction proceeds in the forward and the backward directions.
- N-(9Z-octadecenoyl)-L-asparagine + H2O = L-asparagine + (9Z)-octadecenoateThis reaction proceeds in the forward direction.
- N-(9Z-octadecenoyl)-L-glutamine + H2O = L-glutamine + (9Z)-octadecenoateThis reaction proceeds in the forward direction.
- N-(9Z-octadecenoyl)-L-leucine + H2O = L-leucine + (9Z)-octadecenoateThis reaction proceeds in the forward and the backward directions.
- N-(9Z-octadecenoyl)-L-lysine + H2O = L-lysine + (9Z)-octadecenoateThis reaction proceeds in the forward direction.
- N-(9Z-octadecenoyl)-L-methionine + H2O = (9Z)-octadecenoate + L-methionineThis reaction proceeds in the forward direction.
- N-(9Z-octadecenoyl)-L-serine + H2O = L-serine + (9Z)-octadecenoateThis reaction proceeds in the forward direction.
- N-(9Z-octadecenoyl)-L-tryptophan + H2O = L-tryptophan + (9Z)-octadecenoateThis reaction proceeds in the forward direction.
- N-(9Z-octadecenoyl)-L-tyrosine + H2O = L-tyrosine + (9Z)-octadecenoateThis reaction proceeds in the forward direction.
- N-hexadecanoyl-L-phenylalanine + H2O = hexadecanoate + L-phenylalanineThis reaction proceeds in the forward direction.
- N-octadecanoyl-L-phenylalanine + H2O = octadecanoate + L-phenylalanineThis reaction proceeds in the forward direction.
Pathway
Amino-acid metabolism.
Lipid metabolism; fatty acid metabolism.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides | |
Molecular Function | metal ion binding | |
Molecular Function | peptidase activity | |
Biological Process | amide biosynthetic process | |
Biological Process | amide catabolic process | |
Biological Process | amino acid metabolic process | |
Biological Process | cellular lipid metabolic process | |
Biological Process | proteolysis |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Submitted names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Chondrichthyes > Holocephali > Chimaeriformes > Callorhinchidae > Callorhinchus
Accessions
- Primary accessionA0A4W3GJ87
Proteomes
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-28 | |||||
Sequence: MQVPGWLRIVFLAVSLLCCALLLRTLSA | ||||||
Chain | PRO_5021276083 | 29-506 | ||||
Sequence: GGGRKVDRLRAASAADFTPGENERLLSNLRAAVQIPTVSLARAQPDTAAMEAFRDYLPQAFPGVFSSDLVRHEIIANYSHLFTVRGSDPGLRPYILTAHLDVVPVAEEKWDFPPFSGEESDGYLYGRGTLDDKASAVGILQALEFLLNRGYVPRRAFYVAIGHDEEVGGDNGARNIAATLESRGVKLSFLLDEGSVILDHIIPGLKKPVAVIAVTEKGQALFSLKVNTQTGHSSMPMRESSIGILASAVSRLEKKPMPRMFGLGPELAMFEQLGPEMSFPLNIIMANLWLFAPIISRTLEKNPSMNALVRTTTAVTEFHAGIKPNVLPPTANATLNFRIHPAHSVQGLIEEMRYTIDDDRVKIDLLYGFDPLPTSPHDELAFGYQIIRTTVENVFPEVFVIPGLSVANTDTRHYQHLTEGLYRFVPLWITQEDTGRIHGPNERISVKNYEEIVRFYFHLIQNSDLNLRLNVHSSGHEL |
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 243-384 | Peptidase M20 dimerisation | ||||
Sequence: TEKGQALFSLKVNTQTGHSSMPMRESSIGILASAVSRLEKKPMPRMFGLGPELAMFEQLGPEMSFPLNIIMANLWLFAPIISRTLEKNPSMNALVRTTTAVTEFHAGIKPNVLPPTANATLNFRIHPAHSVQGLIEEMRYTI |
Sequence similarities
Belongs to the peptidase M20A family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length506
- Mass (Da)56,087
- Last updated2019-09-18 v1
- ChecksumBFB042E256A8A9C5
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A4W3GKN8 | A0A4W3GKN8_CALMI | PM20D1 | 523 | ||
A0A4W3GJU9 | A0A4W3GJU9_CALMI | PM20D1 | 508 | ||
A0A4W3H0R0 | A0A4W3H0R0_CALMI | PM20D1 | 495 |
Keywords
- Technical term