A0A4V3ZPU9 · A0A4V3ZPU9_9GAMM

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site305Mg2+ (UniProtKB | ChEBI); catalytic
Binding site348Mg2+ (UniProtKB | ChEBI); catalytic
Binding site406Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site409Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • ORF names
      E8Q33_02340

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • SB9B
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Thiotrichales > Piscirickettsiaceae > Methylophaga

Accessions

  • Primary accession
    A0A4V3ZPU9

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain39-122S1 motif
Region406-409Required for zinc-mediated homotetramerization and catalytic activity
Region564-811Disordered
Compositional bias571-593Basic and acidic residues
Compositional bias640-654Basic and acidic residues
Compositional bias743-757Polar residues
Compositional bias758-811Basic and acidic residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    811
  • Mass (Da)
    90,230
  • Last updated
    2019-07-31 v1
  • Checksum
    0C2CFA4C2D8C552E
MKRILINATHEEELRVAMVDGQRLFDLDIDTPSREQKKGNIYKGKITRVEPSLEAVFVEYGSERQGFLPLKEISKTYFRQHKDDGEGNGRINVQDVLSVGQELVVQIEKEERGNKGAALTTFISLAGRYLVLMPNSPRAGGISRRIEGDDRAELQEALRELEIPEGMGMIVRTAGVGKQTEELQWDLEYLVQLWTAIDLATKDRSAPFLVYQESNIIIRALRDYLRKDIGEILVDSPEVYQTGYDFMRMVMPHELNKFKLYQDKVPLFTRYQLESQIESAFRHEVRLPSGGALVIDPTEALISIDVNSARANKGGDIEETALNTNLEAAEEIARQLRLRDLGGLVVIDFIDMGPTRNQREVENRLRDHLKSDRARVQIGRISRFGLLEMSRQRLRPALGDAHQEVCPRCAGLGHVRGVESLGLAVLRLVEEEATKDNVSQLVVQLPVSVATFMLNEKRAQIDAIEKRHGVRLLLIPNPHLETPHYDIERIKDGNEKQTSSHQLISTPEIEAAVVLKEKPVMEQPAVTGIAPIAPAPIIKPTAAVQPERKPSLLKRLLGVLTGSPVEEPVEASKPRTAEKESDAEREARNQNRRSRGRNNSNRRNNNRPRRPEGVAGEELTTAKAAAPSAANVVEAENKPAVDANGEEKSRNPNAKRSRRGGRRRRGRGEGDVATATDTTVNTANETVEKVERVERGNAIPTPPPEVNGNAIPAPEPEINGNAIPVSTEPEIDGNKIPEKSAPRRSRTSTSASTNNRRRRGAPPKDKENREANEVEKNSASADNETQKVEKPKPVEKPKPTEESPKADAKES

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias571-593Basic and acidic residues
Compositional bias640-654Basic and acidic residues
Compositional bias743-757Polar residues
Compositional bias758-811Basic and acidic residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
SSXS01000002
EMBL· GenBank· DDBJ
THK42412.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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