A0A4U0ZMT7 · A0A4U0ZMT7_9BACT

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site45L-glutamine (UniProtKB | ChEBI)
Binding site238L-glutamine (UniProtKB | ChEBI)
Binding site240L-glutamine (UniProtKB | ChEBI)
Active site265Nucleophile
Binding site266L-glutamine (UniProtKB | ChEBI)
Binding site269L-glutamine (UniProtKB | ChEBI)
Binding site307L-glutamine (UniProtKB | ChEBI)
Binding site309L-glutamine (UniProtKB | ChEBI)
Binding site310L-glutamine (UniProtKB | ChEBI)
Active site350
Active site352

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      FCL48_20570

Organism names

  • Taxonomic identifier
  • Strain
    • IMCC35007
  • Taxonomic lineage
    Bacteria > Thermodesulfobacteriota > Desulfobulbia > Desulfobulbales > Desulfocapsaceae > Desulforhopalus

Accessions

  • Primary accession
    A0A4U0ZMT7

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain1-131Carbamoyl-phosphate synthase small subunit N-terminal
Region1-189CPSase

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    374
  • Mass (Da)
    41,024
  • Last updated
    2019-07-31 v1
  • Checksum
    28E8972D1E719476
MKAIIALEDGTTFEGHSFTGHGEAVGEIVFNTSLSGYQEILTDPSYTGQIVTMTYPLIGNYGTNPEDMESAAAHPKAFLIKEYNSYPSNYRSTKPLAEFLQEYGILGVEGFDTRALVRHIRTKGAMKGIVSTRDLDKEALVKKAQEWSGLVGQDMVKKVTCTSPYGWGENEPVAGSNFETAKKPGEKAFKVVAFDFGIKYNQLRLLQKKGCQVQVVPARTDAKTVLAMEPDGIFLSNGPGDPEGVEGVVDTIRELLGKKPIFGICLGHQMLGLAYGGSAYKLKFGHRGGNQPVKDLTTGHVEITSQNHGFCIDESTLPAGEVEVTHINLNDNSLEGMRHKKYPAFSVQYHPEHAPGPHDAEYLFDRFIDMMRAV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
SWCN01000025
EMBL· GenBank· DDBJ
TKB06527.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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