A0A4R8EEF5 · A0A4R8EEF5_9BACT
- Protein2-isopropylmalate synthase
- GeneleuA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids510 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-ketoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).
Catalytic activity
- 3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H+
Cofactor
Pathway
Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 13 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 201 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 203 | Mn2+ (UniProtKB | ChEBI) | |||
Binding site | 237 | Mn2+ (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | 2-isopropylmalate synthase activity | |
Molecular Function | acetyl-CoA C-acetyltransferase activity | |
Molecular Function | manganese ion binding | |
Biological Process | L-leucine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name2-isopropylmalate synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Thermotogota > Thermotogae > Petrotogales > Petrotogaceae > Petrotoga
Accessions
- Primary accessionA0A4R8EEF5
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 4-264 | Pyruvate carboxyltransferase | |||
Region | 391-510 | Regulatory domain | |||
Sequence similarities
Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length510
- Mass (Da)56,563
- Last updated2019-07-31 v1
- Checksum092354C00F80CDDF
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
SODZ01000021 EMBL· GenBank· DDBJ | TDX10160.1 EMBL· GenBank· DDBJ | Genomic DNA |