A0A4R5L815 · A0A4R5L815_9BURK
- ProteinAdenosylmethionine-8-amino-7-oxononanoate aminotransferase
- GenebioA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids456 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the transfer of the alpha-amino group from S-adenosyl-L-methionine (SAM) to 7-keto-8-aminopelargonic acid (KAPA) to form 7,8-diaminopelargonic acid (DAPA). It is the only aminotransferase known to utilize SAM as an amino donor.
Catalytic activity
- (8S)-8-amino-7-oxononanoate + S-adenosyl-L-methionine = (7R,8S)-7,8-diammoniononanoate + S-adenosyl-4-methylsulfanyl-2-oxobutanoate
Cofactor
Pathway
Cofactor biosynthesis; biotin biosynthesis; 7,8-diaminononanoate from 8-amino-7-oxononanoate (SAM route): step 1/1.
Features
Showing features for site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 29 | Participates in the substrate recognition with KAPA and in a stacking interaction with the adenine ring of SAM | ||||
Sequence: C | ||||||
Binding site | 66 | substrate | ||||
Sequence: W | ||||||
Binding site | 126-127 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | ||||
Sequence: GA | ||||||
Binding site | 159 | substrate | ||||
Sequence: Y | ||||||
Binding site | 265 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 294 | substrate | ||||
Sequence: K | ||||||
Binding site | 328 | substrate | ||||
Sequence: S | ||||||
Binding site | 329-330 | pyridoxal 5'-phosphate (UniProtKB | ChEBI) | ||||
Sequence: HS | ||||||
Binding site | 418 | substrate | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | adenosylmethionine-8-amino-7-oxononanoate transaminase activity | |
Molecular Function | pyridoxal phosphate binding | |
Biological Process | biotin biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAdenosylmethionine-8-amino-7-oxononanoate aminotransferase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Paraburkholderia
Accessions
- Primary accessionA0A4R5L815
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 294 | N6-(pyridoxal phosphate)lysine | ||||
Sequence: K |
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length456
- Mass (Da)50,362
- Last updated2019-07-31 v1
- ChecksumAA881AF5D1BE09C1