A0A4R5L3T5 · A0A4R5L3T5_9BURK

Function

function

Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O2 and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress.

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 1 FAD per subunit.
heme b (UniProtKB | Rhea| CHEBI:60344 )

Note: Binds 1 heme b (iron(II)-protoporphyrin IX) group per subunit.

Features

Showing features for site, binding site, active site.

TypeIDPosition(s)Description
Site29Involved in heme-bound ligand stabilization and O-O bond activation
Site84Influences the redox potential of the prosthetic heme and FAD groups
Binding site85Fe (UniProtKB | ChEBI) of heme b (UniProtKB | ChEBI); proximal binding residue
Active site95Charge relay system
Active site137Charge relay system
Binding site190FAD (UniProtKB | ChEBI)
Binding site206-209FAD (UniProtKB | ChEBI)
Binding site275-280NADP+ (UniProtKB | ChEBI)
Site394Influences the redox potential of the prosthetic heme and FAD groups
Binding site395-398FAD (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionFAD binding
Molecular Functionheme binding
Molecular Functionmetal ion binding
Molecular Functionnitric oxide dioxygenase NAD(P)H activity
Molecular Functionoxygen binding
Molecular Functionoxygen carrier activity
Biological Processcellular response to nitrosative stress
Biological Processnitric oxide catabolic process
Biological Processresponse to toxic substance

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Flavohemoprotein
  • Alternative names
    • Flavohemoglobin
    • Hemoglobin-like protein
    • Nitric oxide dioxygenase
      (NO oxygenase
      ; NOD
      ) (EC:1.14.12.17
      ) . EC:1.14.12.17 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      hmpA
    • Synonyms
      hmp
    • ORF names
      E1N52_37885

Organism names

  • Taxonomic identifier
  • Strain
    • CNPSo 3008
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Betaproteobacteria > Burkholderiales > Burkholderiaceae > Paraburkholderia

Accessions

  • Primary accession
    A0A4R5L3T5

Proteomes

Family & Domains

Features

Showing features for domain, region.

TypeIDPosition(s)Description
Domain9-133Globin
Region149-403Reductase
Domain152-262FAD-binding FR-type

Domain

Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H.

Sequence similarities

Belongs to the globin family. Two-domain flavohemoproteins subfamily.
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    403
  • Mass (Da)
    44,449
  • Last updated
    2019-07-31 v1
  • Checksum
    DFAB8636898D881B
MLTQRTKDIVKATAPVLADHGYAIIKRFYTRLFEAHPELKNVFNMAHQEQGQQQQALARAVYAYAENIEDPSSLAAVLKNIANKHASLGVLPEHYPIVGEHLLGAIKEVLGDAATEDIISAWALAYGNLADVLMGMESELYEGSADKLGGWTGWRKFVVREKRPESSIITSFVLEPADGGPVANFEPGQYISIGVNVPTLGLQQIRQYSLSDMPNGRTYRISVKREAGGPQPAGYVSNLLHDHVNVGDEVKLAAPYGNFHIDVNAHTPIVLISGGVGLTPMISMLKRALQDPNRKVVFVHGARNSGVHAMRDRLRETAATNANFHLEIFYNEPLPGDVQGRDYNYAGFVDIGQIKQAILQPDADYYICGPIPFMRMQHDALKNLGVHEARIHYEVFGPDLFAE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
SMOD01000052
EMBL· GenBank· DDBJ
TDG02818.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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