A0A4R3RYP5 · A0A4R3RYP5_9HYPH

  • Protein
    tRNA-dihydrouridine(20/20a) synthase
  • Gene
    dusA
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    3/5

Function

function

Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Cofactor

FMN (UniProtKB | Rhea| CHEBI:58210 )

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site143FMN (UniProtKB | ChEBI)
Site170Interacts with tRNA
Active site173Proton donor
Binding site212FMN (UniProtKB | ChEBI)
Binding site244FMN (UniProtKB | ChEBI)
Site256Interacts with tRNA; defines subfamily-specific binding signature
Site259Interacts with tRNA
Binding site284-286FMN (UniProtKB | ChEBI)
Binding site306-307FMN (UniProtKB | ChEBI)
Site372Interacts with tRNA; defines subfamily-specific binding signature
Site375Interacts with tRNA; defines subfamily-specific binding signature

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular FunctionFMN binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA-dihydrouridine20 synthase activity
Molecular FunctiontRNA-dihydrouridine20a synthase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-dihydrouridine(20/20a) synthase
  • EC number
  • Alternative names
    • U20-specific dihydrouridine synthase
      (U20-specific Dus
      )
    • tRNA-dihydrouridine synthase A

Gene names

    • Name
      dusA
    • ORF names
      EV129_102114

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • IE4868
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Rhizobiaceae > Rhizobium/Agrobacterium group > Rhizobium

Accessions

  • Primary accession
    A0A4R3RYP5

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain88-391DUS-like FMN-binding

Sequence similarities

Belongs to the Dus family. DusA subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    410
  • Mass (Da)
    45,102
  • Last updated
    2019-07-31 v1
  • Checksum
    74D6C39CD54BE74A
MISEAIPHNAHACPVADATIFSLARGDSSHDRILIWYMAIPVGALCQVRRRNRRIWHKTSVNKTEKKLLQAPEKKEKGNYFKAPVFAVAPMIDWTDRHCRYFHRQISRNALLYTEMVVADAIIHGPRERLLSFDGDEHPVALQLGGSDPVKLAEALRIAGPYGYDEVNLNVGCPSDRVQSGTFGACLMLTPKVVANCVMAMKAVSNVPVTVKCRIGVDEQEPEEALPELLARVLDAGADAVWIHARKAWLKGLSPKENREIPPLDYDIVYRMKERWPEVFIGINGGILTLDEAAAHLKHVDGVMLGRAAYQNAGILADLDHRFFGAPAAEPDWDGLRDRMMAYAARHLESGGRLQHVARHMVGLFTGLPGARRYRQILSSDAAKAGAGPEVIAAAFAAVDFPGVKEALSA

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
SMBK01000002
EMBL· GenBank· DDBJ
TCU39977.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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