A0A4Q5E3T5 · A0A4Q5E3T5_BACUN
- ProteinATP-dependent dethiobiotin synthetase BioD
- GenebioD
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids215 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.
Catalytic activity
- (7R,8S)-7,8-diammoniononanoate + ATP + CO2 = (4R,5S)-dethiobiotin + ADP + 3 H+ + phosphate
Cofactor
Pathway
Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 15-20 | ATP (UniProtKB | ChEBI) | ||||
Sequence: DAGKSY | ||||||
Binding site | 19 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Active site | 40 | |||||
Sequence: K | ||||||
Binding site | 44 | substrate | ||||
Sequence: T | ||||||
Binding site | 52 | ATP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 52 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 117 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 117-120 | ATP (UniProtKB | ChEBI) | ||||
Sequence: EGAG | ||||||
Binding site | 213 | ATP (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | dethiobiotin synthase activity | |
Molecular Function | magnesium ion binding | |
Biological Process | biotin biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameATP-dependent dethiobiotin synthetase BioD
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacteroidota > Bacteroidia > Bacteroidales > Bacteroidaceae > Bacteroides
Accessions
- Primary accessionA0A4Q5E3T5
Proteomes
Subcellular Location
Interaction
Subunit
Homodimer.
Structure
Sequence
- Sequence statusComplete
- Length215
- Mass (Da)24,473
- Last updated2019-07-31 v1
- Checksum52D8C5DF406DF122