A0A4P8DJE5 · DMXL3_CRYX8
- ProteinCytochrome P450 monooxygenase dmxL3
- GenedmxL3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids514 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of the dimeric xanthones cryptosporioptides (PubMed:30996871).
The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase dmx-nrPKS (Probable). The atrochrysone carboxyl ACP thioesterase dmxR1 then breaks the thioester bond and releases the atrochrysone carboxylic acid from dmx-nrPKS (Probable). Atrochrysone carboxylic acid is decarboxylated by the decarboxylase dmxR15, and oxidized by the anthrone oxygenase dmxR16 to yield emodin (Probable). Emodin is then reduced to emodin hydroquinone by the oxidoreductase dmxR7 (Probable). A-ring reduction by the short chain dehydrogenase dmxR18, dehydration by the scytalone dehydratase-like protein dmxR17 and probable spontaneous re-oxidation, results in overall deoxygenation to chrysophanol (PubMed:30996871).
Baeyer-Villiger oxidation by the Baeyer-Villiger monooxygenase (BVMO) dmxR6 then yields monodictylactone in equilibrium with monodictyphenone (PubMed:30996871).
In the case of the cryptosporioptides biosynthesis, monodictylactone is reduced at C-12 to an alcohol (by the short chain dehydrogenases dmxR12 or dmxR8) and hydroxylated at C-5 by dmxR9, yielding the electron-rich aromatic which could eliminate H2O to form the ortho-quinonemethide, followed by tautomerisation to paraquinone and complete the formal reduction to produce the 10-methylgroup (Probable). Conjugate addition of C-4a-OH to the resulting paraquinone by the monooxygenase dmxR10 then gives cyclohexadienone, which is then reduced at C-5 by the short chain dehydrogenase dmxR3 to give the dihydroxanthone (Probable). The 6,7-epoxide in the cryptosporioptides could be introduced by the cytochrome P450 monooxygenase dmxL3 (Probable). The highly reducing PKS dmxL2 manufactures butyrate, which is further carboxylated by dmxL1 to form ethylmalonate (PubMed:30996871).
It is not yet clear whether the carboxylation occurs while the butyrate is attached to the ACP of dmxL2, but this unusual fungal metabolite could then be esterified to O-5 by the O-acetyltransferase dmxR13 (PubMed:30996871).
Finally, dimerization performed by dmxR5 gives the observed dimers cryptosporioptides A, B and C as the final products of the pathway (PubMed:30996871).
The pathway begins with the synthesis of atrochrysone thioester by the polyketide synthase dmx-nrPKS (Probable). The atrochrysone carboxyl ACP thioesterase dmxR1 then breaks the thioester bond and releases the atrochrysone carboxylic acid from dmx-nrPKS (Probable). Atrochrysone carboxylic acid is decarboxylated by the decarboxylase dmxR15, and oxidized by the anthrone oxygenase dmxR16 to yield emodin (Probable). Emodin is then reduced to emodin hydroquinone by the oxidoreductase dmxR7 (Probable). A-ring reduction by the short chain dehydrogenase dmxR18, dehydration by the scytalone dehydratase-like protein dmxR17 and probable spontaneous re-oxidation, results in overall deoxygenation to chrysophanol (PubMed:30996871).
Baeyer-Villiger oxidation by the Baeyer-Villiger monooxygenase (BVMO) dmxR6 then yields monodictylactone in equilibrium with monodictyphenone (PubMed:30996871).
In the case of the cryptosporioptides biosynthesis, monodictylactone is reduced at C-12 to an alcohol (by the short chain dehydrogenases dmxR12 or dmxR8) and hydroxylated at C-5 by dmxR9, yielding the electron-rich aromatic which could eliminate H2O to form the ortho-quinonemethide, followed by tautomerisation to paraquinone and complete the formal reduction to produce the 10-methylgroup (Probable). Conjugate addition of C-4a-OH to the resulting paraquinone by the monooxygenase dmxR10 then gives cyclohexadienone, which is then reduced at C-5 by the short chain dehydrogenase dmxR3 to give the dihydroxanthone (Probable). The 6,7-epoxide in the cryptosporioptides could be introduced by the cytochrome P450 monooxygenase dmxL3 (Probable). The highly reducing PKS dmxL2 manufactures butyrate, which is further carboxylated by dmxL1 to form ethylmalonate (PubMed:30996871).
It is not yet clear whether the carboxylation occurs while the butyrate is attached to the ACP of dmxL2, but this unusual fungal metabolite could then be esterified to O-5 by the O-acetyltransferase dmxR13 (PubMed:30996871).
Finally, dimerization performed by dmxR5 gives the observed dimers cryptosporioptides A, B and C as the final products of the pathway (PubMed:30996871).
Cofactor
Pathway
Secondary metabolite biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 459 | Fe (UniProtKB | ChEBI) of heme (UniProtKB | ChEBI); axial binding residue | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | membrane | |
Molecular Function | heme binding | |
Molecular Function | iron ion binding | |
Molecular Function | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCytochrome P450 monooxygenase dmxL3
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Leotiomycetes > Helotiales > Dermateaceae > Cryptosporiopsis
Accessions
- Primary accessionA0A4P8DJE5
Subcellular Location
UniProt Annotation
GO Annotation
Membrane ; Single-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Transmembrane | 1-21 | Helical | |||
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000453475 | 1-514 | Cytochrome P450 monooxygenase dmxL3 | ||
Structure
Sequence
- Sequence statusComplete
- Length514
- Mass (Da)58,013
- Last updated2019-07-31 v1
- ChecksumAC89C11F1C5D0848
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MK182094 EMBL· GenBank· DDBJ | QCL09088.1 EMBL· GenBank· DDBJ | Genomic DNA |