A0A4D8XRT0 · A0A4D8XRT0_9ALVE
- ProteinMultifunctional fusion protein
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids854 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln).
Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in the mitochondria. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).
Catalytic activity
- L-aspartyl-tRNA(Asn) + L-glutamine + ATP + H2O = L-asparaginyl-tRNA(Asn) + L-glutamate + ADP + phosphate + 2 H+
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 60 | Charge relay system | ||||
Sequence: S | ||||||
Active site | 84 | Acyl-ester intermediate | ||||
Sequence: S |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | glutamyl-tRNA(Gln) amidotransferase complex | |
Cellular Component | mitochondrion | |
Molecular Function | ATP binding | |
Molecular Function | glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity | |
Biological Process | glutaminyl-tRNAGln biosynthesis via transamidation | |
Biological Process | mitochondrial translation |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameMultifunctional fusion protein
Including 2 domains:
- Recommended nameGlutamyl-tRNA(Gln) amidotransferase subunit B, mitochondrial
- EC number
- Short namesGlu-AdT subunit B
- Recommended nameGlutamyl-tRNA(Gln) amidotransferase subunit A, mitochondrial
- EC number
- Short namesGlu-AdT subunit A
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Sar > Alveolata > Perkinsozoa > Perkinsea > Perkinsida > Perkinsidae > Perkinsus
Accessions
- Primary accessionA0A4D8XRT0
Proteomes
Subcellular Location
Interaction
Subunit
Heterotrimer of A, B and C subunits.
Subunit of the heterotrimeric GatCAB amidotransferase (AdT) complex, composed of A, B and C subunits.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 703-853 | Asn/Gln amidotransferase | ||||
Sequence: DFYEAVVTAAGGAANGKIAANWVMGEFSGALNKAGLDLADSPVSTEKLGGMIARIVDNTISGKIAKQVFGFMWEEGKTADEIIAEKGLKQETDTGAIEAIIKEVLAANEKMVEEYKSGKEKAFNGLVGQVMKASRGKANPAQVNELMKKLI |
Sequence similarities
Belongs to the GatB/GatE family. GatB subfamily.
Belongs to the amidase family. GatA subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length854
- Mass (Da)92,773
- Last updated2019-07-03 v1
- ChecksumD0B129B4C79C4602
Keywords
- Technical term