A0A4D7QNB9 · A0A4D7QNB9_9HYPH

Function

function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
    EC:5.6.2.1 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site, active site.

1905100200300400500600700800900
Type
IDPosition(s)Description
Site31Interaction with DNA
Site141Interaction with DNA
Site142Interaction with DNA
Site145Interaction with DNA
Site157Interaction with DNA
Active site303O-(5'-phospho-DNA)-tyrosine intermediate
Site305Interaction with DNA
Site507Interaction with DNA

GO annotations

AspectTerm
Cellular Componentchromosome
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type I (single strand cut, ATP-independent) activity
Molecular Functionmetal ion binding
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA topoisomerase 1
  • EC number
  • Alternative names
    • DNA topoisomerase I

Gene names

    • Name
      topA
    • ORF names
      E8L99_18715

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • NMCR1094
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Phreatobacteraceae > Phreatobacter

Accessions

  • Primary accession
    A0A4D7QNB9

Proteomes

Subcellular Location

Interaction

Subunit

Monomer.

Family & Domains

Features

Showing features for domain, region, compositional bias.

Type
IDPosition(s)Description
Domain1-117Toprim
Region165-170Interaction with DNA
Region330-356Disordered
Region849-905Disordered
Compositional bias859-875Basic and acidic residues
Compositional bias876-895Basic residues

Sequence similarities

Belongs to the type IA topoisomerase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    905
  • Mass (Da)
    98,474
  • Last updated
    2019-07-31 v1
  • MD5 Checksum
    2CDAD3E34510E023764EC09BC9C833F2
MNVVIVESPAKAKTINKYLGPDYEVLASFGHVRDLPAKDGSVDPNADFAMIWEADGRGAKRISDIAKAVKGADKLILATDPDREGEAISWHLLEALREKRVLKDMPVERVTFNAITKQAVLEAMANPRAIDVALVDAYLARRALDYLVGFTLSPVLWRKLPGARSAGRVQSVALRLVCERESEIERFVKREYWSLVATLATPRAETFEARLVGADGKKIQRLDVGTETEARDFEKALQEAHFAVANVEAKPARRHPYPPFTTSTLQQEASRKLGFAPNRTMQVAQRLYEGTDIGGETVGLITYMRTDGVDMAPEAVSAVRDMIGKDFGPRYVPGSPRKYQTKQKNAQEAHEAIRPTDARRHPRDVARYLEPEQAKLYELIWKRTVASQMESAELERTTVDVVATVGGLGASLGRKLELRATGQVVKFDGFLTLYTEDRDDESEDEDSKRLPAVTVGEKLDRRTIATSQHFTEPPPRYSEASLVKRMEELGIGRPSTYASTLQTLRDREYVTIDKKKLVPEDKGRLVIAFLENFFDRYVEYGFTAALEEKLDLVSNSELDWKALLRDFWKDFSAAIDQTKELRVSDVLSALNESLGPHIFPDRGDGSNPRVCPTCGTGMLSLKLGKFGAFIGCSNYPECRFTRQLAAPSADAVDGPAGASADGTRKLGADPVSGLEVTVRDGRFGPYVQLGEGEKPKRSSLPKGMTPGGIDLERALALLELPKQVATHPESGNPIQVGIGRYGPYVQHEKTYANLEKDDDILAIGANRAIDLIIAKEQGGGRGRFGRAAAAPGRAIGDHPSLGGPITVMAGKYGPYVKHGSVNATLPKGKNPDELTMDEAVALIAARAEAVGTSGKKPARRPAAKADGDKASAKKTAAKKAPAKKAAAKKPAAKKPAAKKAAGGEG

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias859-875Basic and acidic residues
Compositional bias876-895Basic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP039865
EMBL· GenBank· DDBJ
QCK88865.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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