A0A4D7H9D1 · A0A4D7H9D1_9EURY
- ProteinDNA double-strand break repair Rad50 ATPase
- Generad50
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids897 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. Rad50 controls the balance between DNA end bridging and DNA resection via ATP-dependent structural rearrangements of the Rad50/Mre11 complex.
Cofactor
Note: Binds 1 zinc ion per homodimer.
Features
Showing features for binding site.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | zinc ion binding | |
Biological Process | double-strand break repair |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameDNA double-strand break repair Rad50 ATPase
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageArchaea > Euryarchaeota > Stenosarchaea group > Halobacteria > Halobacteriales > Haloferacaceae > Haloprofundus
Accessions
- Primary accessionA0A4D7H9D1
Proteomes
Interaction
Subunit
Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain, coiled coil.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 339-354 | Polar residues | ||||
Sequence: KAQGFENQSSNLGEKA | ||||||
Region | 339-386 | Disordered | ||||
Sequence: KAQGFENQSSNLGEKATDLESRAEQKRTRADELEAERETAEAELDDRR | ||||||
Compositional bias | 355-386 | Basic and acidic residues | ||||
Sequence: TDLESRAEQKRTRADELEAERETAEAELDDRR | ||||||
Domain | 410-509 | Zinc-hook | ||||
Sequence: RGDASEHLADLREQKSSLRDELETVRTKLRSTRQRVEEARRLLDEGKCPECGQPVDESPHVDTLDEDRARVDDLDDEREELEAEVASLDDDIDTAAELVD | ||||||
Region | 456-490 | Disordered | ||||
Sequence: KCPECGQPVDESPHVDTLDEDRARVDDLDDEREEL | ||||||
Compositional bias | 512-655 | Basic and acidic residues | ||||
Sequence: NELERLRERRDSVTTLVDQRERTLAEKAEQAEAARTAAEEHEGKAEELREAAEQTASRAEETRDRIAELEEEREGVKSSEERLERIQSLRDEISDAESKLDRLSERRSDLDELNDERRERLAEKQRRRRELREDVDESQIRQAK | ||||||
Region | 512-662 | Disordered | ||||
Sequence: NELERLRERRDSVTTLVDQRERTLAEKAEQAEAARTAAEEHEGKAEELREAAEQTASRAEETRDRIAELEEEREGVKSSEERLERIQSLRDEISDAESKLDRLSERRSDLDELNDERRERLAEKQRRRRELREDVDESQIRQAKEQRSNAE | ||||||
Coiled coil | 679-733 | |||||
Sequence: RTKLDNDIGGAKTDIERLEELQEKRDHVEERVDALESLHEETEELEAMYGDLRAE | ||||||
Region | 873-897 | Disordered | ||||
Sequence: PTSNRSTVRHEEAAGLSAVESVADD |
Domain
The two conserved Cys that bind zinc constitute the zinc-hook, which separates the large intramolecular coiled coil regions. The 2 Cys residues coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another Rad50 molecule, thereby forming a V-shaped homodimer.
Sequence similarities
Belongs to the SMC family. RAD50 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length897
- Mass (Da)101,880
- Last updated2019-07-03 v1
- Checksum5C9D636E87F33E92
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 339-354 | Polar residues | ||||
Sequence: KAQGFENQSSNLGEKA | ||||||
Compositional bias | 355-386 | Basic and acidic residues | ||||
Sequence: TDLESRAEQKRTRADELEAERETAEAELDDRR | ||||||
Compositional bias | 512-655 | Basic and acidic residues | ||||
Sequence: NELERLRERRDSVTTLVDQRERTLAEKAEQAEAARTAAEEHEGKAEELREAAEQTASRAEETRDRIAELEEEREGVKSSEERLERIQSLRDEISDAESKLDRLSERRSDLDELNDERRERLAEKQRRRRELREDVDESQIRQAK |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP039833 EMBL· GenBank· DDBJ | QCJ47660.1 EMBL· GenBank· DDBJ | Genomic DNA |