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A0A4D6YIA8 · A0A4D6YIA8_9GAMM

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

Type
IDPosition(s)Description
Binding site51L-glutamine (UniProtKB | ChEBI)
Binding site245L-glutamine (UniProtKB | ChEBI)
Binding site247L-glutamine (UniProtKB | ChEBI)
Active site273Nucleophile
Binding site274L-glutamine (UniProtKB | ChEBI)
Binding site277L-glutamine (UniProtKB | ChEBI)
Binding site315L-glutamine (UniProtKB | ChEBI)
Binding site318L-glutamine (UniProtKB | ChEBI)
Active site357
Active site359

GO annotations

AspectTerm
Cellular Componentcarbamoyl-phosphate synthase complex
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • ORF names
      D9V77_00715

Organism names

Accessions

  • Primary accession
    A0A4D6YIA8

Proteomes

Subcellular Location

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Family & Domains

Features

Showing features for region, domain.

Type
IDPosition(s)Description
Region1-196CPSase
Domain7-137Carbamoyl-phosphate synthase small subunit N-terminal

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    386
  • Mass (Da)
    42,859
  • Last updated
    2019-07-31 v1
  • MD5 Checksum
    E9E81F59125F567DB09F0F64FF832A76
MEGVLSQSAILVLEDGTRFHGRAIGAKGITVGEVVFNTSITGYQEIITDPSYSHQIVTLTYPHIGNVGSNLDDEESSKIYIKGLIIRDLSLIASNYRNKKSFSCYLKENNIIAISDIDTRKLTRILRTKGSQNGCIIEDKKENYTIGHNKAKNFMSLQGLDLAKKVSTQSIYNWSQGSFSLNKNKFVSIEKEKLLFHVVVYDFGVKRNILRMLVDRGCYLTIVPATTDPKTVLNLSPDGIFLSNGPGDPRPCHYAIDAIRYFLKTNIPIFGICLGHQLLALAVGAEIIKMKFGHHGGNHPVKEIKNNRVIITSQNHSFTVDIKNLPNNIEITHSSLFDGTLQGLSLTNKSAFSFQGHPEASPGPHDASSLFDTFIKLIAYEKTRTQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP034855
EMBL· GenBank· DDBJ
QCI25368.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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