A0A4D6XY52 · A0A4D6XY52_9GAMM

Function

function

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

  • NAD+ + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide.
    EC:6.5.1.2 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site32-36NAD+ (UniProtKB | ChEBI)
Binding site81-82NAD+ (UniProtKB | ChEBI)
Binding site113NAD+ (UniProtKB | ChEBI)
Active site115N6-AMP-lysine intermediate
Binding site136NAD+ (UniProtKB | ChEBI)
Binding site173NAD+ (UniProtKB | ChEBI)
Binding site291NAD+ (UniProtKB | ChEBI)
Binding site315NAD+ (UniProtKB | ChEBI)
Binding site409Zn2+ (UniProtKB | ChEBI)
Binding site412Zn2+ (UniProtKB | ChEBI)
Binding site433Zn2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionDNA binding
Molecular FunctionDNA ligase (NAD+) activity
Molecular Functionmetal ion binding
Biological ProcessDNA repair
Biological ProcessDNA replication

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA ligase
  • EC number
  • Alternative names
    • Polydeoxyribonucleotide synthase [NAD(+)]

Gene names

    • Name
      ligA
    • ORF names
      D9V72_00335

Organism names

Accessions

  • Primary accession
    A0A4D6XY52

Proteomes

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain596-677BRCT

Sequence similarities

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    677
  • Mass (Da)
    77,886
  • Last updated
    2019-07-31 v1
  • Checksum
    A35EDF559ED07D7F
MTSTKNKIDKLRKNILKYEYFYHTLDESIVSDAEYDYLLHQLYNLELKNKELITSDSPTQKIGANLLSKFKKIKHFFPMLSLENTFDINGYLTFEKRIKKLINIDVAVSLCCELKIDGIAISIIYEEGILVQAATRGDGFQGENVTANARMIESIPLNLKGVDIPKRLEVRGEVFMLKSDFIKLNQNNKMNQNKIFSNPRNAAAGSLRHINPKITAKRKLRFSCYGCYFFKENNEELTTHYKRLMRCSSWGLPVNKDILLCSNYIEVIEYYKKFEKKRKSLEFDIDGIVIKVNSIELQNKLGFNTKSPRWAIAFKFFSAERITILNDVKFQVGRTGSITPVAYFDPVYISGVMISKASLHNKSEIERLDLHAHDSVIICRSGDVIPRVLGVIQTIRCKNAKKIVFPKFCPVCNTQLLENIEEKIIRCHAGLTCDAQKQKSLHHFFSKKSLYVIGLGPKIINELIQKGLVKNPIDFFYLKNIDLTKLKNVGNKKSINIINSINKCKQTTFKRFIYALGIPGVGDTVSEKIANYFTKLDELINADILKLNDIDGVGKVISNNIFNYFSIISNRNMVNELIIKVGIFWNNQDRYELNNIKKTYFFHKKIVLTGVFKSFSRMELKRILINLGAKILNNISQKTDCLIYGKNFGSKFFKAKNLNIRMINEEELESLILIEKN

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP034867
EMBL· GenBank· DDBJ
QCI22532.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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