A0A4D6Q9B0 · FL3H1_CROXC
- ProteinFlavanone 3-dioxygenase F3H1
- GeneF3H-1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids372 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the 3-beta-hydroxylation of (2S)-flavanones to 2R,3R-dihydroflavonols, which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants (PubMed:31004005).
Can act on naringenin to produce dihydrokaempferol, and on eriodictyol to produced dihydroquercetin (PubMed:31004005).
Can act on naringenin to produce dihydrokaempferol, and on eriodictyol to produced dihydroquercetin (PubMed:31004005).
Catalytic activity
- a (2S)-flavan-4-one + 2-oxoglutarate + O2 = a (2R,3R)-dihydroflavonol + succinate + CO2This reaction proceeds in the forward direction.
- (2S)-naringenin + 2-oxoglutarate + O2 = (2R,3R)-dihydrokaempferol + succinate + CO2This reaction proceeds in the forward direction.
- (S)-eriodictyol + 2-oxoglutarate + O2 = (2R,3R)-dihydroquercetin + succinate + CO2This reaction proceeds in the forward direction.
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 ascorbate molecule per subunit.
Note: Binds 1 Fe2+ ion per subunit.
Pathway
Flavonoid metabolism.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 222 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 224 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 280 | Fe cation (UniProtKB | ChEBI) | |||
Binding site | 290 | 2-oxoglutarate (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | flavanone 3-dioxygenase activity | |
Molecular Function | L-ascorbic acid binding | |
Molecular Function | metal ion binding | |
Biological Process | flavonoid biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameFlavanone 3-dioxygenase F3H1
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > Liliopsida > Asparagales > Iridaceae > Crocoideae > Freesieae > Crocosmia
Accessions
- Primary accessionA0A4D6Q9B0
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000448071 | 1-372 | Flavanone 3-dioxygenase F3H1 | ||
Expression
Tissue specificity
Expressed in young cromes.
Structure
Sequence
- Sequence statusComplete
- Length372
- Mass (Da)41,370
- Last updated2019-07-03 v1
- Checksum98603F9E589A20AF
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MK562522 EMBL· GenBank· DDBJ | QCF41217.1 EMBL· GenBank· DDBJ | mRNA |