A0A4D6IA61 · KABC_DIGSM
- ProteinAlpha-ketoglutarate dependent kainoid synthase
- GenekabC
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids360 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Iron/ascorbate-dependent oxidoreductase: part of the gene cluster that mediates the biosynthesis of kainic acid (KA) and derivatives, natural products with neurochemical activity acting as ionotropic glutamate receptor (iGluR) agonists, thus being neurotoxins (PubMed:30995339).
Catalyzes the conversion of prekainic acid to kainic acid and kainic acid lactone (PubMed:30995339).
Catalyzes the conversion of prekainic acid to kainic acid and kainic acid lactone (PubMed:30995339).
Catalytic activity
- 2-oxoglutarate + O2 + prekainate = CO2 + H2O + kainate + succinateThis reaction proceeds in the forward direction.
- 2-oxoglutarate + H+ + O2 + prekainate = CO2 + H2O + kainate lactone + succinateThis reaction proceeds in the forward direction.
Cofactor
Note: Binds 1 Fe2+ ion per subunit.
Activity regulation
Inhibited by the iron chelator EDTA.
Biotechnology
Escherichia coli cells expressing Digenea simplex kabC are successfully converting supplied synthetic prekainic acid into kainic acid, thus providing a less expensive method to produce this molecule for neuro-pharmacological applications.
Pathway
Secondary metabolite biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 2-oxoglutarate-dependent dioxygenase activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAlpha-ketoglutarate dependent kainoid synthase
- EC number
- Short namesAlpha-KG dependent kainoid synthase
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Rhodophyta > Florideophyceae > Rhodymeniophycidae > Ceramiales > Rhodomelaceae > Polysiphonioideae > Digenea
Accessions
- Primary accessionA0A4D6IA61
Phenotypes & Variants
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000454272 | 1-360 | Alpha-ketoglutarate dependent kainoid synthase | |||
Sequence: MTVSKYNGVASTFNGVARRFDFAPLEADKLNWYPRSALPPEIPAIDLNKVNTEEELAQFLVDIRKSGLFYIVDHGIPEEISIGCYNAFREFCNLPEEAREKYNTDESFKSGGYVPFKGTSIGGGNLFERQKDFVVKFFWRGPSVVNRSPNDRFAEFHDEHHRKTAELAEKIITTILKALKTRFPEFHPDELKDNINVRNMFFSNRIYPEAPPDDGEKADYRLVPHRDLSFITLANQVPANNGFKGLFILTGDGEKIPVPPIRNSYLVFIGQGLSYLTNKYLPAALHGVDFPDNTNFEGSERASLISFYEPNDYMMPSKNINPLPEEIFEKSCTFYDDVGVGRAGTTYNYVRYKFHEGYYL |
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 200-310 | Fe2OG dioxygenase | ||||
Sequence: MFFSNRIYPEAPPDDGEKADYRLVPHRDLSFITLANQVPANNGFKGLFILTGDGEKIPVPPIRNSYLVFIGQGLSYLTNKYLPAALHGVDFPDNTNFEGSERASLISFYEP |
Sequence similarities
Belongs to the iron/ascorbate-dependent oxidoreductase family.
Family and domain databases
Sequence
- Sequence statusComplete
- Length360
- Mass (Da)41,163
- Last updated2019-07-03 v1
- ChecksumBD06A2E1FAAD0F22