A0A4C9B5I3 · A0A4C9B5I3_ECOLX
- ProteinProtein/nucleic acid deglycase HchA
- GenehchA
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids283 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of Schiff bases and advanced glycation endproducts (AGE). Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Plays an important role in protecting cells from carbonyl stress.
Catalytic activity
- N2-(1-hydroxy-2-oxoethyl)-GDP + H2O = glycolate + GDP + H+
- N2-(1-hydroxy-2-oxoethyl)-GMP + H2O = glycolate + GMP + H+
- N2-(1-hydroxy-2-oxoethyl)-GTP + H2O = glycolate + GTP + H+
- N2-(1-hydroxy-2-oxoethyl)-dGTP + H2O = dGTP + glycolate + H+
- N2-(1-hydroxy-2-oxopropyl)-GDP + H2O = lactate + GDP + H+
- N2-(1-hydroxy-2-oxopropyl)-GMP + H2O = lactate + GMP + H+
- N2-(1-hydroxy-2-oxopropyl)-GTP + H2O = lactate + GTP + H+
- N2-(1-hydroxy-2-oxopropyl)-dGTP + H2O = lactate + dGTP + H+
- N6-(1-hydroxy-2-oxoethyl)-L-lysyl-[protein] + H2O = glycolate + L-lysyl-[protein] + H+
- N(omega)-(1-hydroxy-2-oxoethyl)-L-arginyl-[protein] + H2O = L-arginyl-[protein] + glycolate + H+
- S-(1-hydroxy-2-oxoethyl)-L-cysteinyl-[protein] + H2O = glycolate + L-cysteinyl-[protein] + H+
- an N2-(1-hydroxy-2-oxoethyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + glycolate + H+
- an N2-(1-hydroxy-2-oxoethyl)-guanosine in RNA + H2O = a guanosine in RNA + glycolate + H+
- an N2-(1-hydroxy-2-oxopropyl)-2'-deoxyguanosine in DNA + H2O = a 2'-deoxyguanosine in DNA + lactate + H+
- an N2-(1-hydroxy-2-oxopropyl)-guanosine in RNA + H2O = a guanosine in RNA + lactate + H+
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 86 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 91 | Zn2+ (UniProtKB | ChEBI) | |||
Binding site | 123 | Zn2+ (UniProtKB | ChEBI) | |||
Active site | 185 | Nucleophile | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | glyoxalase III activity | |
Molecular Function | protein deglycase activity | |
Molecular Function | thiolester hydrolase activity | |
Molecular Function | zinc ion binding | |
Biological Process | DNA repair | |
Biological Process | methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione | |
Biological Process | protein repair |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein/nucleic acid deglycase HchA
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionA0A4C9B5I3
Proteomes
Subcellular Location
Expression
Induction
By heat shock.
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Sequence similarities
Belongs to the peptidase C56 family. HSP31-like subfamily.
Belongs to the peptidase C56 family. HchA subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length283
- Mass (Da)31,263
- Last updated2019-07-31 v1
- Checksum5E78A12C12E93B72
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
JABWMK020000050 EMBL· GenBank· DDBJ | MBB2468624.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CABWJB010000001 EMBL· GenBank· DDBJ | VWM87336.1 EMBL· GenBank· DDBJ | Genomic DNA |