A0A484YH89 · A0A484YH89_9ENTR

Function

function

A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA.

Miscellaneous

In the RecBCD complex, RecB has a slow 3'-5' helicase, an exonuclease activity and loads RecA onto ssDNA, RecD has a fast 5'-3' helicase activity, while RecC stimulates the ATPase and processivity of the RecB helicase and contributes to recognition of the Chi site.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site23-30ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function3'-5' DNA helicase activity
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionDNA binding
Molecular Functionexodeoxyribonuclease V activity
Molecular Functionisomerase activity
Biological Processdouble-strand break repair via homologous recombination

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    RecBCD enzyme subunit RecB
  • EC number
  • Alternative names
    • DNA 3'-5' helicase subunit RecB
    • Exonuclease V subunit RecB
      (ExoV subunit RecB
      )
    • Helicase/nuclease RecBCD subunit RecB

Gene names

    • Name
      recB_2
    • Synonyms
      recB
    • ORF names
      NCTC12126_03492

Organism names

  • Taxonomic identifier
  • Strain
    • NCTC12126
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Enterobacter > Enterobacter cloacae complex

Accessions

  • Primary accession
    A0A484YH89

Proteomes

Interaction

Subunit

Heterotrimer of RecB, RecC and RecD. All subunits contribute to DNA-binding. Interacts with RecA.

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-764DNA-binding and helicase activity, interacts with RecC
Domain2-450UvrD-like helicase ATP-binding
Domain480-746UvrD-like helicase C-terminal
Region764-778Nuclease activity, interacts with RecD and RecA

Domain

The C-terminal domain has nuclease activity and interacts with RecD. It interacts with RecA, facilitating its loading onto ssDNA.
The N-terminal DNA-binding domain is a ssDNA-dependent ATPase and has ATP-dependent 3'-5' helicase function. This domain interacts with RecC.

Sequence similarities

Belongs to the helicase family. UvrD subfamily.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    778
  • Mass (Da)
    88,139
  • Last updated
    2019-06-05 v1
  • Checksum
    480B74A49AC6029E
MTDTAESLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGLGGNAAFPRPLSVEELLVVTFTEAATAELRGRIRANIHELRIACLRQTTDNPLYASLLEEIADKQQAAQWLLLAERQMDDASVFTIHGFCQRMLSLNAFESGMLFEQQLIEDESELRYQACADFWRRHCYPLPRDIAEAVHALWKGPEDLLRAIGRYLQGEAPVIKSPPPADETLASRHEKIIAQIVGLKQKWNESVGDIDAIIENSGIDRRKFNRGNQGKWIEKISAWAQEETRGYQLPDALEKFSQRFLAERTKADGIVPEHPLFVSIEALLAEPLSLNDLIITRAMAEIRDAVAREKRRRGELGFDDMLSRLDEALRSENGEALAHAIRTRFPVAMIDEFQDTDPQQYRIFRRIWRQQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVEAHYTLDTNWRSSPGMVESVNALFERMDAAFMFREIPFKPVKSASKNADLRFEYNGAVQPAMRFWLLDGEGYGVVDHQTAMAQHCAAQIRDWLSAGSRGEALLWKGENATPVKASDITVLVRSRQEAALIRDALTLLTIPSVYLSNRDSVFDTLEAQEMLWLLQAVLAPERESTLRSALASSMLGLNARDIDALNNNETAWDEVVDEFVHYRERWQKRGVMAMLRELMAQRRIAENLLATAGGERRLTDILHISELLQEAGSQLESEHALVRWLSQQIADPNSNASSQQMRLESDKHLVQIVTIHKSKGLEYPLVWLPFIANYRVQDQAFYHDRETFGGGA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CAADIW010000029
EMBL· GenBank· DDBJ
VFS35598.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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