A0A484CWB7 · A0A484CWB7_PERFV
- ProteinPhosphoinositide phospholipase C
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids744 (go to sequence)
- Protein existencePredicted
- Annotation score3/5
Function
Catalytic activity
- a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + H2O = 1D-myo-inositol 1-phosphate + a 1,2-diacyl-sn-glycerol + H+This reaction proceeds in the forward direction.
Cofactor
Note: Binds 3 Ca2+ ions per subunit. Two of the Ca2+ ions are bound to the C2 domain.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 304 | |||||
Sequence: H | ||||||
Binding site | 305 | Ca2+ 3 (UniProtKB | ChEBI); catalytic | ||||
Sequence: N | ||||||
Binding site | 334 | Ca2+ 3 (UniProtKB | ChEBI); catalytic | ||||
Sequence: E | ||||||
Binding site | 336 | Ca2+ 3 (UniProtKB | ChEBI); catalytic | ||||
Sequence: D | ||||||
Active site | 349 | |||||
Sequence: H | ||||||
Binding site | 383 | Ca2+ 3 (UniProtKB | ChEBI); catalytic | ||||
Sequence: E | ||||||
Binding site | 432 | substrate | ||||
Sequence: K | ||||||
Binding site | 434 | substrate | ||||
Sequence: K | ||||||
Binding site | 508 | substrate | ||||
Sequence: S | ||||||
Binding site | 535 | substrate | ||||
Sequence: R | ||||||
Binding site | 637 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: I | ||||||
Binding site | 639 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 663 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 692 | Ca2+ 5 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 694 | Ca2+ 5 (UniProtKB | ChEBI) | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum | |
Cellular Component | nucleus | |
Molecular Function | calcium ion binding | |
Molecular Function | phosphatidylinositol phospholipase C activity | |
Biological Process | intracellular signal transduction | |
Biological Process | lipid catabolic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphoinositide phospholipase C
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Actinopterygii > Neopterygii > Teleostei > Neoteleostei > Acanthomorphata > Eupercaria > Perciformes > Percoidei > Percidae > Percinae > Perca
Accessions
- Primary accessionA0A484CWB7
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Features
Showing features for glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Glycosylation | 184 | O-linked (GlcNAc) serine | ||||
Sequence: S |
Keywords
- PTM
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 15-123 | PH | ||||
Sequence: KVMMAGSTLKKVKSRSWKKQRHFRLLEDAVTIWYKSRWAGKGHSTFSISEVEAVREGHQSEVLQSTAEEFPAELCFTLVFHGRQGNLDLVAKTPEEAKAWIEGVRKLIH | ||||||
Domain | 133-168 | EF-hand | ||||
Sequence: RQDQWIRDWFQKADKNKDGKMNFKEVQTLLKMMNVE | ||||||
Domain | 169-204 | EF-hand | ||||
Sequence: MNEDHALNLFTKADKSESGSLEIEQFVHFYKMLTQR | ||||||
Region | 441-473 | Disordered | ||||
Sequence: DCLDETQTDEVSDEEAEDAESPTKGNPPARKKS | ||||||
Compositional bias | 443-457 | Acidic residues | ||||
Sequence: LDETQTDEVSDEEAE | ||||||
Compositional bias | 458-473 | Basic and acidic residues | ||||
Sequence: DAESPTKGNPPARKKS | ||||||
Domain | 479-595 | PI-PLC Y-box | ||||
Sequence: LSDLVVYCKSVHFRDFEHARSHAKCYEISSFSENKAQARAKDEGTDFVRYNTRQLSRIYPDGLRTNSSNYNPQEMWNVGCQIVALNFQTAGLEMDLNDGLFRQNGCCGYVLKPDFMR | ||||||
Domain | 595-723 | C2 | ||||
Sequence: RDGNTQFSPDKPEERQGYKPLRLSIQVISGQQLPKVNQKEGSIVDPLVRVQIFGVPGDQDMKETPYINNNGFNPVWDETLNFVIHTPELALVRFEVEDHDRASRNDFIGQFTLPFMCIQAGYRHIHLLS |
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length744
- Mass (Da)85,140
- Last updated2019-06-05 v1
- ChecksumA893D037D491BECE
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 443-457 | Acidic residues | ||||
Sequence: LDETQTDEVSDEEAE | ||||||
Compositional bias | 458-473 | Basic and acidic residues | ||||
Sequence: DAESPTKGNPPARKKS |
Keywords
- Technical term