A0A482N8M8 · ICCE_TALVA
- ProteinNADH-dependent flavin oxidoreductase iccE
- GeneiccE
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids414 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
NADH-dependent flavin oxidoreductase; part of the gene cluster that mediates the biosynthesis of ilicicolin H, a 4-hydroxy-2-pyridonealkaloid that has potent and broad antifungal activities by inhibiting the mitochondrial respiration chain (PubMed:30905148).
IccE acts as an epimerase and catalyzes the conversion of 8-epi-ilicicolin H into the final product ilicicolin H (PubMed:30905148).
The biosynthesis of ilicicolin H starts with formation of the tetramic acid by the hybrid PKS-NRPS synthetase iccA with the partnering trans-enoyl reductase iccB since iccA lacks a designated enoylreductase (ER) domain. The cytochrome P450 monooxygenase iccC then catalyzes the ring expansion of the tetramate to the acyclic 2-pyridone. The pericyclase iccD further converts the acyclic 2-pyridone into 8-epi-ilicicolin H. Finally, the epimerase iccE converts 8-epi-ilicicolin H into ilicicolin H via epimerization. IccA to iccE are sufficient for ilicicolin H biosynthesis and the roles of the remaining enzymes, iccF, iccG and iccH within the pathway have still to be determined (Probable) (PubMed:30905148).
IccE acts as an epimerase and catalyzes the conversion of 8-epi-ilicicolin H into the final product ilicicolin H (PubMed:30905148).
The biosynthesis of ilicicolin H starts with formation of the tetramic acid by the hybrid PKS-NRPS synthetase iccA with the partnering trans-enoyl reductase iccB since iccA lacks a designated enoylreductase (ER) domain. The cytochrome P450 monooxygenase iccC then catalyzes the ring expansion of the tetramate to the acyclic 2-pyridone. The pericyclase iccD further converts the acyclic 2-pyridone into 8-epi-ilicicolin H. Finally, the epimerase iccE converts 8-epi-ilicicolin H into ilicicolin H via epimerization. IccA to iccE are sufficient for ilicicolin H biosynthesis and the roles of the remaining enzymes, iccF, iccG and iccH within the pathway have still to be determined (Probable) (PubMed:30905148).
Catalytic activity
- 8-epi-ilicicolin H = ilicicolin HThis reaction proceeds in the forward direction.
Pathway
Mycotoxin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 25-28 | FMN (UniProtKB | ChEBI) | |||
Binding site | 107 | FMN (UniProtKB | ChEBI) | |||
Binding site | 188-191 | substrate | |||
Binding site | 347-348 | FMN (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | FMN binding | |
Molecular Function | oxidoreductase activity | |
Molecular Function | polyketide synthase activity | |
Molecular Function | racemase and epimerase activity | |
Biological Process | ilicicolin H biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNADH-dependent flavin oxidoreductase iccE
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Trichocomaceae > Talaromyces
Accessions
- Primary accessionA0A482N8M8
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000449009 | 1-414 | NADH-dependent flavin oxidoreductase iccE | ||
Structure
Sequence
- Sequence statusComplete
- Length414
- Mass (Da)44,593
- Last updated2019-06-05 v1
- Checksum40A328191E224456
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
MK539848 EMBL· GenBank· DDBJ | QBQ83707.1 EMBL· GenBank· DDBJ | Genomic DNA |