A0A482N8M8 · ICCE_TALVA

Function

function

NADH-dependent flavin oxidoreductase; part of the gene cluster that mediates the biosynthesis of ilicicolin H, a 4-hydroxy-2-pyridonealkaloid that has potent and broad antifungal activities by inhibiting the mitochondrial respiration chain (PubMed:30905148).
IccE acts as an epimerase and catalyzes the conversion of 8-epi-ilicicolin H into the final product ilicicolin H (PubMed:30905148).
The biosynthesis of ilicicolin H starts with formation of the tetramic acid by the hybrid PKS-NRPS synthetase iccA with the partnering trans-enoyl reductase iccB since iccA lacks a designated enoylreductase (ER) domain. The cytochrome P450 monooxygenase iccC then catalyzes the ring expansion of the tetramate to the acyclic 2-pyridone. The pericyclase iccD further converts the acyclic 2-pyridone into 8-epi-ilicicolin H. Finally, the epimerase iccE converts 8-epi-ilicicolin H into ilicicolin H via epimerization. IccA to iccE are sufficient for ilicicolin H biosynthesis and the roles of the remaining enzymes, iccF, iccG and iccH within the pathway have still to be determined (Probable) (PubMed:30905148).

Catalytic activity

Pathway

Mycotoxin biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site25-28FMN (UniProtKB | ChEBI)
Binding site107FMN (UniProtKB | ChEBI)
Binding site188-191substrate
Binding site347-348FMN (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionFMN binding
Molecular Functionoxidoreductase activity
Molecular Functionpolyketide synthase activity
Molecular Functionracemase and epimerase activity
Biological Processilicicolin H biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    NADH-dependent flavin oxidoreductase iccE
  • EC number
  • Alternative names
    • Epimerase iliE
    • Ilicicolin H biosynthesis cluster protein E

Gene names

    • Name
      iccE

Organism names

Accessions

  • Primary accession
    A0A482N8M8

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004490091-414NADH-dependent flavin oxidoreductase iccE

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    414
  • Mass (Da)
    44,593
  • Last updated
    2019-06-05 v1
  • Checksum
    40A328191E224456
MADLHLAKSITLRCGLTLPNRLAKTAIAESIAPRNMLIDEGFHRVYAPWAEGGWGMVLTGHVQVDSMHLGTHTDPAVNADFSDDQIVGSWKVWAAVCNRHGTPTIMQLNHPGRQAPIGAGTSGVFAKNISASAVPMDIGSGLLARAVSKIVFGTPREMTVDDIGKLVRQFARAARLASQSGFAGVELHASHGYLLAQFLSAATNRRKDAYGGTPANRARIVVEIIKAVRAEVPASFCVGIILTAVHGSASTEEFQSFIEQAQLICDAGVDFIEISGGTFETPSMFIGPEKRGKAYDWDNQEAFFLDFAQAIRPHLGTVPLLLTGGFRSCHAMEAAVKRGDCDMIGLARPAVVNPLLPKTVVFNPEVNKSGDTKLHATRTPAPWYIKLFGITALNVHMDNAWYVGRLQTLAKTGR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MK539848
EMBL· GenBank· DDBJ
QBQ83707.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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