A0A481Y580 · A0A481Y580_9MONO

  • Protein
    RNA-directed RNA polymerase L
  • Gene
    L
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both activities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.

Catalytic activity

  • GTP + H2O = GDP + H+ + phosphate
  • a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-homocysteine
  • a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + 2 S-adenosyl-L-methionine = a 5'-end (N7-methyl 5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + H+ + 2 S-adenosyl-L-homocysteine
    EC:2.1.1.375 (UniProtKB | ENZYME | Rhea)
  • a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + S-adenosyl-L-methionine = a 5'-end (5'-triphosphoguanosine)-(2'-O-methyladenylyl)-adenylyl-cytidylyl-adenosine in mRNA + H+ + S-adenosyl-L-homocysteine
  • a 5'-end triphospho-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + GDP + H+ = a 5'-end (5'-triphosphoguanosine)-adenylyl-adenylyl-cytidylyl-adenosine in mRNA + diphosphate
    EC:2.7.7.88 (UniProtKB | ENZYME | Rhea)
  • a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)
    EC:2.7.7.48 (UniProtKB | ENZYME | Rhea)

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell cytoplasm
Cellular Componentvirion component
Molecular FunctionATP binding
Molecular FunctionGTPase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular FunctionRNA-dependent RNA polymerase activity

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    RNA-directed RNA polymerase L
  • Short names
    Protein L
  • Alternative names
    • Large structural protein
    • Replicase
    • Transcriptase

Including 5 domains:

  • Recommended name
    RNA-directed RNA polymerase
  • EC number
  • Recommended name
    GTP phosphohydrolase
  • EC number
  • Recommended name
    GDP polyribonucleotidyltransferase
  • EC number
  • Alternative names
    • PRNTase
  • Recommended name
    mRNA (nucleoside-2'-O-)-methyltransferase
  • EC number
  • Short names
    N1-2'-O-MTase
  • Recommended name
    mRNA (guanine-N(7)-)-methyltransferase
  • Short names
    G-N7-MTase

Gene names

    • Name
      L

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • PAR-05
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Negarnaviricota > Haploviricotina > Monjiviricetes > Mononegavirales > Pneumoviridae > Metapneumovirus > Metapneumovirus avis

Accessions

  • Primary accession
    A0A481Y580

Subcellular Location

Keywords

Interaction

Subunit

Interacts with the phosphoprotein (via C-terminus); the association of P and L forms the polymerase complex.

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain627-810RdRp catalytic
Domain1655-1845Mononegavirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the paramyxovirus L protein family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,999
  • Mass (Da)
    229,407
  • Last updated
    2019-06-05 v1
  • Checksum
    464F6D7D329FE7F5
MDLITEEVVNVYLPDSYLKGVISFSETNALGSSILGLPYLKKDNTSHVAIQNPVIEHNRLKYLFNTLKIPKTYNCVEPLMLQNEIMHMGTCIKPHKLKEWLKRAKECSSLRLQMLEDWLQLKNNTDKNLYDCICTDSIPIWVEEWFNTWLILHEHISNYRKMEVSQQGAIYRRNEDKMYYALSSFGCILISKKSKRYTFCTYNQLLTWKDMALSRFNANFCVWVSNCLNNQQEGSGLRSSLSGGLLNKLYHDTDFMLSTTGNECFALVKEFEGFLMSEILHMTEHAQFSLRFRNTLLNGLADKVYKIRPDIMRRREGTVCTETAVMKDDEIKTLLGIILRTIKLLIQSRIENAAELYFIFRIFGHPMVEEREAMDAVKLNNEVTKVLSLKSLTEMRGAFILRIIKGFVATYKRWPRVKNPTTLSRRWQMYRDAKSYPSDLELTLDDYLELAGINFRQEFHIPTKTNLEMVLNDKAISPPKDLIWSVFPKNYLPQNIQDEFRSKCYESKEKNKTRRVLEYYLKDPNFSQEDLKHYVVHQRYLNDRNHVVSLTGKERELSVGRMFAMQPGDQRQVQILAEKMLSDNIVPFFPETLTKYGDLELQRIMELKSELSSTKTRHCDTYNNYIARASIVTDLSKFNQAFRYETTSVCADIADDLHGTQSLFCWLHLTIPMTTVLCTYRHAPPNTYGMYNIDTIPEQSGLYRYHMGGIEGWCQKMWTMEAISLLDVVSVKNRVQLTSLLNGDNQSIDVSKPVRLTANYTEVQADYSLAIQMLREIRDAYKNIGHKLKEGETYVSRDLQFISKIIQSEGVMYPSAIKKILRVGPWINTILDDIKTSMEAIGSLCQELEFRGESILTSTILKNFWLYIQCFVEVKNHSLAGTQLQTELNQTLRSLKLYFKLSSEKKTVNLYLNIPMHLGGGDPVVFYRSFYRRTPDFLTEVISHVSLLLRAAKVDSDITDQFFRSLLNVTKNEKATLTTLMRDPQAIGSERQAKITSDINRTAVTSVLSTAPNQLFSDSALHFSQNEQEIGRVAEGITPTYPHGLRVLYEAFPFYKAEKVVNMISGTKSVTNILQRTSAISGIDIERAVRMMVHNLGLLGRILNTAPSAKQVPKVGDKLLCCKLSQQIREASWDSLEIIGVSSPSMVSCMSLVYTTALEGEGIVIEKFSTERTTRGTRGPKMPWVGSSTQEKKLVPVYNRQILSKVQRDQLETIGKLRWVYKGVTGLRRTLNKICLGTLGLPYKLLKPLLPRFMSVNFLHRLAVSSRPVEFPASIPAYRTTNYHFDTSPINKTLSERFGNEDINLVFQNAISCGVSVMSVVEQLTGRSPKQLVMKPLVEDIDILPPPTFQGVIDHKYVNKIVCDQHIFNPDKITLSDLGKILLPTVQVSALDRGQNYECFFNGTNIVETLSTSLACYWCSTLLQMYAENTIFQKEWGDGFITDHAFVNFEIFMSAFKTYLTCHWQRENYTMITDQNLADKLLRVNNYFWKIMSKVLLTQKVKRRVMIYDINIVNVLSSQSFKSWFIERMNKTHWGEVPWIVNADGDLVEVKASENYLALLNNSTCIKTIMLNYSDMAHAMTRLLRCKNMQDHVPQLTKTISPTDISPVIDPTTTLFIFPKAPYMTMTKFSMDKISSNYTLKRNPMTILPWHHVMRYNSVYSSTGCKLSIKGTLAKLIAKLEVKVIYFVGEGAGHLMARTACEYPSMKFVYRSLKSDDNHCYPIEYARILGGMSRVLDYGDGLSIEATDVTKKDHWEHLHRICKDPLLLTVCDAELKNLNLFMTAIIQWRRHVLSCRICTTYGTNIYLYIKYHAEEVNHMLPHFVKLLAVHIMQNSKLSGSECYVLLGLGHQSKLPGYSEVRGAKAQMSMTGNVSMPIKIDKGAVTMNLRTILPGLKIPITESSVKELIATMREEMPCAPSMTYIGGSRIIERKWVNRKVCNVAKWIVHILNMPKGDPSYDYYEVIENTYPNMMDLLENLTRTEVKKLVKITGYLLTCKK

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
MK491499
EMBL· GenBank· DDBJ
QBK51049.1
EMBL· GenBank· DDBJ
Viral cRNA

Similar Proteins

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