A0A480VC23 · A0A480VC23_PIG
- ProteinPhosphatidylethanolamine N-methyltransferase
- GenePEMT
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids259 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the three sequential steps of the methylation pathway for the biosynthesis of phosphatidylcholine, a critical and essential component for membrane structure. Uses S-adenosylmethionine (S-adenosyl-L-methionine, SAM or AdoMet) as the methyl group donor for the methylation of phosphatidylethanolamine (1,2-diacyl-sn-glycero-3-phosphoethanolamine, PE) to phosphatidylmonomethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine, PMME), PMME to phosphatidyldimethylethanolamine (1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine, PDME), and PDME to phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine, PC), producing S-adenosyl-L-homocysteine in each step.
Catalytic activity
- 1,2-diacyl-sn-glycero-3-phospho-N,N-dimethylethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phosphocholine + H+ + S-adenosyl-L-homocysteine
Pathway
Lipid metabolism.
Phospholipid metabolism; phosphatidylcholine biosynthesis.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | mitochondrial membrane | |
Molecular Function | phosphatidyl-N-dimethylethanolamine N-methyltransferase activity | |
Molecular Function | phosphatidyl-N-methylethanolamine N-methyltransferase activity | |
Molecular Function | phosphatidylethanolamine N-methyltransferase activity | |
Biological Process | methylation | |
Biological Process | phosphatidylcholine biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namePhosphatidylethanolamine N-methyltransferase
- EC number
- Short namesPEAMT ; PEMT
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Suina > Suidae > Sus
Accessions
- Primary accessionA0A480VC23
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Endoplasmic reticulum membrane ; Multi-pass membrane protein
Mitochondrion membrane ; Multi-pass membrane protein
Membrane ; Multi-pass membrane protein
Note: Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria.
Features
Showing features for topological domain, transmembrane, intramembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-72 | Lumenal | ||||
Sequence: MGRHGGRFCGPGAPARPARQPLQGGPRPGAPASEDHAGPGGGARLLRGLRQPGPQADLCVMTRLLGYVDPSD | ||||||
Transmembrane | 73-92 | Helical | ||||
Sequence: ARFVAAVLTIAFNPLFWNVV | ||||||
Intramembrane | 73-93 | Helical | ||||
Sequence: ARFVAAVLTIAFNPLFWNVVA | ||||||
Topological domain | 94-105 | Lumenal | ||||
Sequence: RWEHKTRKLSRA | ||||||
Topological domain | 127-153 | Cytoplasmic | ||||
Sequence: RSHCFTQAMLSQPQMESLGGPTGYRLG | ||||||
Transmembrane | 154-179 | Helical | ||||
Sequence: LALLGVGSVFVLSSFLALGFTGTFLG | ||||||
Topological domain | 175-217 | Lumenal | ||||
Sequence: GTFLGDYFGILMEARVTRFPFSVLDNPMYWGSTAVYLGWALMH | ||||||
Topological domain | 239-259 | Cytoplasmic | ||||
Sequence: FEEPFTAEIYRQRGSQARKRS |
Keywords
- Cellular component
PTM/Processing
Proteomic databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-44 | Disordered | ||||
Sequence: MGRHGGRFCGPGAPARPARQPLQGGPRPGAPASEDHAGPGGGAR |
Sequence similarities
Belongs to the class VI-like SAM-binding methyltransferase superfamily. PEMT/PEM2 methyltransferase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length259
- Mass (Da)27,981
- Last updated2019-06-05 v1
- ChecksumA312ACFC6F352EA9
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q7YRH5 | Q7YRH5_PIG | PEMT | 199 | ||
A0A5G2QX89 | A0A5G2QX89_PIG | PEMT | 267 |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DQIR01219262 EMBL· GenBank· DDBJ | HDB74739.1 EMBL· GenBank· DDBJ | Transcribed RNA | ||
DQIR01226080 EMBL· GenBank· DDBJ | HDB81557.1 EMBL· GenBank· DDBJ | Transcribed RNA | ||
DQIR01252154 EMBL· GenBank· DDBJ | HDC07632.1 EMBL· GenBank· DDBJ | Transcribed RNA | ||
DQIR01256029 EMBL· GenBank· DDBJ | HDC11507.1 EMBL· GenBank· DDBJ | Transcribed RNA |