A0A455WF70 · A0A455WF70_MARNT
- ProteinAcireductone dioxygenase
- GenemtnD
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids186 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway.
Catalytic activity
- 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + O2 = 3-(methylsulfanyl)propanoate + CO + formate + 2 H+
Cofactor
Protein has several cofactor binding sites:
Note: Binds 1 Fe2+ cation per monomer.
Note: Binds 1 nickel ion per monomer.
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 5/6.
Features
Showing features for site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Site | 95 | May play a role in metal incorporation in vivo | ||||
Sequence: E | ||||||
Binding site | 96 | Fe2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 96 | Ni2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 98 | Fe2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 98 | Ni2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Site | 101 | May play a role in transmitting local conformational changes | ||||
Sequence: D | ||||||
Binding site | 102 | Fe2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 102 | Ni2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Site | 104 | Important to generate the dianion | ||||
Sequence: R | ||||||
Binding site | 140 | Fe2+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 140 | Ni2+ (UniProtKB | ChEBI) | ||||
Sequence: H |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | acireductone dioxygenase (Ni2+-requiring) activity | |
Molecular Function | acireductone dioxygenase [iron(II)-requiring] activity | |
Molecular Function | iron ion binding | |
Molecular Function | nickel cation binding | |
Biological Process | L-methionine salvage from methylthioadenosine | |
Biological Process | L-methionine salvage from S-adenosylmethionine |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAcireductone dioxygenase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Pseudomonadales > Marinobacteraceae > Marinobacter
Accessions
- Primary accessionA0A455WF70
Interaction
Subunit
Monomer.
Structure
Sequence
- Sequence statusComplete
- Length186
- Mass (Da)21,160
- Last updated2019-06-05 v1
- ChecksumA120D9A82C00F27B