A0A455MDT2 · A0A455MDT2_9ACTN
- ProteinType I polyketide synthase
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids4177 (go to sequence)
- Protein existencePredicted
- Annotation score5/5
Function
function
Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.
Catalytic activity
- (2E)-butenoyl-[ACP] + H+ + NADPH = butanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-decenoyl-[ACP] + H+ + NADPH = decanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-dodecenoyl-[ACP] + H+ + NADPH = dodecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-hexadecenoyl-[ACP] + H+ + NADPH = hexadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-hexenoyl-[ACP] + H+ + NADPH = hexanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- (2E)-octadecenoyl-[ACP] + H+ + NADPH = NADP+ + octadecanoyl-[ACP]This reaction proceeds in the forward direction.
- (2E)-octenoyl-[ACP] + H+ + NADPH = NADP+ + octanoyl-[ACP]This reaction proceeds in the forward direction.
- (2E)-tetradecenoyl-[ACP] + H+ + NADPH = NADP+ + tetradecanoyl-[ACP]This reaction proceeds in the forward direction.
- (3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- (3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2OThis reaction proceeds in the forward direction.
- 3-oxobutanoyl-[ACP] + H+ + NADPH = (3R)-hydroxybutanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxodecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxydecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxododecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxydodecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxohexadecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyhexadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxohexanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyhexanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxooctadecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyoctadecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxooctanoyl-[ACP] + H+ + NADPH = (3R)-hydroxyoctanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- 3-oxotetradecanoyl-[ACP] + H+ + NADPH = (3R)-hydroxytetradecanoyl-[ACP] + NADP+This reaction proceeds in the forward direction.
- H+ + hexadecanoyl-[ACP] + malonyl-[ACP] = 3-oxooctadecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- H+ + hexanoyl-[ACP] + malonyl-[ACP] = 3-oxooctanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- H+ + malonyl-[ACP] + octanoyl-[ACP] = 3-oxodecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- H+ + malonyl-[ACP] + tetradecanoyl-[ACP] = 3-oxohexadecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- acetyl-[ACP] + H+ + malonyl-[ACP] = 3-oxobutanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- butanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxohexanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- decanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxododecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
- dodecanoyl-[ACP] + H+ + malonyl-[ACP] = 3-oxotetradecanoyl-[ACP] + CO2 + holo-[ACP]This reaction proceeds in the forward direction.
Pathway
Lipid metabolism.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 613 | Proton acceptor; for dehydratase activity | ||||
Sequence: H | ||||||
Active site | 780 | Proton donor; for dehydratase activity | ||||
Sequence: D | ||||||
Active site | 2710 | Proton acceptor; for dehydratase activity | ||||
Sequence: H | ||||||
Active site | 2876 | Proton donor; for dehydratase activity | ||||
Sequence: D |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | plasma membrane | |
Molecular Function | (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity | |
Molecular Function | 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity | |
Molecular Function | 3-oxoacyl-[acyl-carrier-protein] synthase activity | |
Molecular Function | [acyl-carrier-protein] S-acetyltransferase activity | |
Molecular Function | [acyl-carrier-protein] S-malonyltransferase activity | |
Molecular Function | enoyl-[acyl-carrier-protein] reductase (NADPH) activity | |
Molecular Function | fatty acid synthase activity | |
Molecular Function | fatty acyl-[ACP] hydrolase activity | |
Molecular Function | phosphopantetheine binding | |
Molecular Function | zinc ion binding | |
Biological Process | DIM/DIP cell wall layer assembly | |
Biological Process | fatty acid biosynthetic process | |
Biological Process | toxin biosynthetic process |
Keywords
- Molecular function
- Ligand
Names & Taxonomy
Protein names
- Submitted names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Catenulisporales > Catenulisporaceae > Catenulispora
Accessions
- Primary accessionA0A455MDT2
Subcellular Location
UniProt Annotation
GO Annotation
PTM/Processing
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 57-99 | Disordered | ||||
Sequence: DGAGAAAEPTDAAVGSGGAAAARADSSPSGAGGTGAAAEPTDV | ||||||
Region | 581-705 | N-terminal hotdog fold | ||||
Sequence: HPLLGAGLTVADSGGVVLAGRWAVGAQSWLADHVVGGVVLVPGTAFLEVVVRAGDEVGCGVVEELVLAAPLVLPASGGVEVQVVVDPDDGSGLRGVGVYARPVGSLGGEWTRHASGSIRQDSDTT | ||||||
Domain | 581-857 | PKS/mFAS DH | ||||
Sequence: HPLLGAGLTVADSGGVVLAGRWAVGAQSWLADHVVGGVVLVPGTAFLEVVVRAGDEVGCGVVEELVLAAPLVLPASGGVEVQVVVDPDDGSGLRGVGVYARPVGSLGGEWTRHASGSIRQDSDTTRVDVGELSVWPPAGAKAIELDGFYDILTGSGFGYGPVFQGLRAVWQGSDGVVFAEVALPEGAPASGFGLHPAVLDAALHAVVFAGLAEAKRGRLPFVWSGVRLVASGASALRVAMRAVGGDAVSLTVADGSGAPVALIESLTLREMADPAAV | ||||||
Region | 720-857 | C-terminal hotdog fold | ||||
Sequence: AKAIELDGFYDILTGSGFGYGPVFQGLRAVWQGSDGVVFAEVALPEGAPASGFGLHPAVLDAALHAVVFAGLAEAKRGRLPFVWSGVRLVASGASALRVAMRAVGGDAVSLTVADGSGAPVALIESLTLREMADPAAV | ||||||
Domain | 1679-1754 | Carrier | ||||
Sequence: TLLLDLVRAQAAAVLGYPNPDTITPSQAFRDLGFDSLTAVELRNRLGGVTGLRLPATLLFDYPSAEALAGHLAIEL | ||||||
Domain | 1786-2214 | Ketosynthase family 3 (KS3) | ||||
Sequence: DEPIAIVSIGCRFPGAVRSPEDLWDLVLNGGDAVSGFPVNRGWDLESLFDDDPDAPGTCYVREGGFLHDAAEFDPVFFGISPREALSMDPQQRLLLETTWEAMERAGLDPEALKGSATGVFVGTSGQDYIATLAASEVVDEGYIGTGNTAAVMSGRISYTFGFEGPAVTVDTACSSSLVALHQAVAALRAGECSLALAGGVTVMSTPNTFIGFSRQRGLALDGRCKAFSDDADGTIWAEGIGMLLLERLSDAQLNGHQVLAVVRGSAVNQDGASNGLTAPNGPSQQRVIRAALADAGVEAADVDLVEAHGTGTSLGDPIEAQALLATYGRNRDGEREPLWLGSIKSNIGHTQAAAGIAGVIKTVMALRNEVMPPTLHVAQPSTHVDWTTGAVELLTAARPWTAGDGAPRRAGVSAFGISGTNAHVILEQ | ||||||
Region | 2675-2802 | N-terminal hotdog fold | ||||
Sequence: HPLLDTVIVAADSGGVVLAGRWAGGSASSQSWLADHVVGGAVLVPGTAFLEVVVRAGDEVGCGVVEELVLAAPLVLPASGGVEVQVVVDPDDGSGLRGVGVFARPVGSLGGEWTRHASGSVRQNSASD | ||||||
Domain | 2675-2953 | PKS/mFAS DH | ||||
Sequence: HPLLDTVIVAADSGGVVLAGRWAGGSASSQSWLADHVVGGAVLVPGTAFLEVVVRAGDEVGCGVVEELVLAAPLVLPASGGVEVQVVVDPDDGSGLRGVGVFARPVGSLGGEWTRHASGSVRQNSASDAEAASLEIWPPADAEAVALDGFYEVLAGSGFGYGPVFQGLKAVWLGADGAVFAEVALPEGTGASGFGLHPAVLDAALHAVTFAGLSETKRGRLPFVWSGVRLIASGASALRVALRAVAGDAVSLTVADGAGAPVAVIESLTLREMADPAAV | ||||||
Region | 2816-2953 | C-terminal hotdog fold | ||||
Sequence: AEAVALDGFYEVLAGSGFGYGPVFQGLKAVWLGADGAVFAEVALPEGTGASGFGLHPAVLDAALHAVTFAGLSETKRGRLPFVWSGVRLIASGASALRVALRAVAGDAVSLTVADGAGAPVAVIESLTLREMADPAAV | ||||||
Domain | 3776-3851 | Carrier | ||||
Sequence: TLILDLVRAQAAAVLGYSDADTITPDRSLPEIGFDSLTAVELRNRLGSATGLRLRSTLVFDHPTPESLAAHLVSEL |