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A0A452SK13 · A0A452SK13_URSAM

Function

function

Fatty acid synthetase is a multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids starting from acetyl-CoA and malonyl-CoA in the presence of NADPH. This multifunctional protein contains 7 catalytic activities and a site for the binding of the prosthetic group 4'-phosphopantetheine of the acyl carrier protein ([ACP]) domain.

Catalytic activity

  • (2E)-decenoyl-[ACP] + NADPH + H+ = decanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-dodecenoyl-[ACP] + NADPH + H+ = dodecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-hexadecenoyl-[ACP] + NADPH + H+ = hexadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-hexenoyl-[ACP] + NADPH + H+ = hexanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-octadecenoyl-[ACP] + NADPH + H+ = octadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-octenoyl-[ACP] + NADPH + H+ = octanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (2E)-tetradecenoyl-[ACP] + NADPH + H+ = tetradecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • (3R)-hydroxybutanoyl-[ACP] = (2E)-butenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxydecanoyl-[ACP] = (2E)-decenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxydodecanoyl-[ACP] = (2E)-dodecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyhexadecanoyl-[ACP] = (2E)-hexadecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyhexanoyl-[ACP] = (2E)-hexenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyoctadecanoyl-[ACP] = (2E)-octadecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxyoctanoyl-[ACP] = (2E)-octenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • (3R)-hydroxytetradecanoyl-[ACP] = (2E)-tetradecenoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
  • 3-oxobutanoyl-[ACP] + NADPH + H+ = (3R)-hydroxybutanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxodecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxydecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxododecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxydodecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxohexadecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyhexadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxohexanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyhexanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxooctadecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyoctadecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxooctanoyl-[ACP] + NADPH + H+ = (3R)-hydroxyoctanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • 3-oxotetradecanoyl-[ACP] + NADPH + H+ = (3R)-hydroxytetradecanoyl-[ACP] + NADP+
    This reaction proceeds in the forward direction.
  • hexadecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxooctadecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • hexanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxooctanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • octanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxodecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • tetradecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxohexadecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • hexadecanoyl-[ACP] + H2O = hexadecanoate + holo-[ACP] + H+
    This reaction proceeds in the forward direction.
    EC:3.1.2.14 (UniProtKB | ENZYME | Rhea)
  • tetradecanoyl-[ACP] + H2O = tetradecanoate + holo-[ACP] + H+
    This reaction proceeds in the forward direction.
    EC:3.1.2.14 (UniProtKB | ENZYME | Rhea)
  • a (3R)-hydroxyacyl-[ACP] + NADP+ = a 3-oxoacyl-[ACP] + NADPH + H+
    This reaction proceeds in the backward direction.
    EC:1.1.1.100 (UniProtKB | ENZYME | Rhea)
  • a (3R)-hydroxyacyl-[ACP] = a (2E)-enoyl-[ACP] + H2O
    This reaction proceeds in the forward direction.
    EC:4.2.1.59 (UniProtKB | ENZYME | Rhea)
  • a 2,3-saturated acyl-[ACP] + NADP+ = a (2E)-enoyl-[ACP] + NADPH + H+
    This reaction proceeds in the backward direction.
    EC:1.3.1.39 (UniProtKB | ENZYME | Rhea)
  • a fatty acyl-[ACP] + malonyl-[ACP] + H+ = a 3-oxoacyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
    EC:2.3.1.41 (UniProtKB | ENZYME | Rhea)
  • holo-[ACP] + acetyl-CoA = acetyl-[ACP] + CoA
    This reaction proceeds in the forward direction.
    EC:2.3.1.38 (UniProtKB | ENZYME | Rhea)
  • acetyl-CoA + n malonyl-CoA + 2n NADPH + 2n H+ = a long-chain fatty acid + (n+1) CoA + n CO2 + 2n NADP+.
    EC:2.3.1.85 (UniProtKB | ENZYME | Rhea)
  • acetyl-[ACP] + malonyl-[ACP] + H+ = 3-oxobutanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • butanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxohexanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • decanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxododecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • dodecanoyl-[ACP] + malonyl-[ACP] + H+ = 3-oxotetradecanoyl-[ACP] + holo-[ACP] + CO2
    This reaction proceeds in the forward direction.
  • holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA
    This reaction proceeds in the forward direction.
    EC:2.3.1.39 (UniProtKB | ENZYME | Rhea)

Pathway

Lipid metabolism; fatty acid biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site882Proton acceptor; for dehydratase activity
Active site1039Proton donor; for dehydratase activity

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Function(3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
Molecular Function3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Function3-oxoacyl-[acyl-carrier-protein] synthase activity
Molecular Function[acyl-carrier-protein] S-acetyltransferase activity
Molecular Function[acyl-carrier-protein] S-malonyltransferase activity
Molecular Functionenoyl-[acyl-carrier-protein] reductase (NADPH) activity
Molecular Functionfatty acid synthase activity
Molecular Functionfatty acyl-[ACP] hydrolase activity
Molecular Functionphosphopantetheine binding
Biological Processfatty acid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

Gene names

    • Name
      FASN

Organism names

Accessions

  • Primary accession
    A0A452SK13

Proteomes

Subcellular Location

PTM/Processing

Keywords

Family & Domains

Features

Showing features for domain, region.

Type
IDPosition(s)Description
Domain1-406Ketosynthase family 3 (KS3)
Region847-980N-terminal hotdog fold
Domain847-1116PKS/mFAS DH
Region990-1116C-terminal hotdog fold
Domain2115-2192Carrier

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    2,494
  • Mass (Da)
    270,614
  • Last updated
    2019-05-08 v1
  • MD5 Checksum
    E7D9C9CF2E9E46302AB2E99C3502D2D2
MEEVVIAGMSGKLPESDNLQEFWANLIGGVDMVTDDDRRWKAGLYGLPRRSGKLKDLSKFDASFFGVHPKQAHTMDPQLRLLLEVTYEAILDGGINPASLRGTHTGVWVGVSGSEASEALSRDPETLLGYSMVGCQRAMMANRISFFFDFKGPSVALDTACSSSLVALQNAYQAIRHGECPCAIVGGINILLKPNTSVQFLKLGMLSPEGSCKSFDESGNGYCRSEAVVVMLLTKKSLARRVYATILNAGINTDGCKEQGVTFPSGEAQEQLIRSLYEPAGLAPESLEYIEAHGTGTKVGDPQELNGIDRALCTTRRDPLLIGSTKSNMGHPEPASGLAALAKVLLSLENGVWAPNLHFHSPNPEIPALREGRLQVVDRPLPVRGGNVGINSFGFGGSNVHVILRPNLQPPSPSTPHVTLPRLLQASGRTPEAVRCLLEQGHQHGRDAAFVSMLNDIAASPPSAMPFRGYTVLGAEDSSLELQEVQQPPNGRRPLWFICSGMGTQWRGMGSSLMRLDSFRDSILRSDEAVKPLGLQVSKLLLSADETTFDDLIHSFVCLTAIQIALIDLLTSMDLRPDGIIGHSLGEVACGYADGCLSQEEAVLTAYWRGQCIKEANIPPGAMAAVGLSWEECKQRCPPGIVPACHNSEDTVTISGPQAEMAAFMEQLKQEGVFAKEVRTGGMAFHSYFMESIAPTLLRALKKVIREPKPRSARWLSTSIPEAQWKGSLARMCSAEYSVNNLVSPVLFQEALCHVPEHAVVLEIAPHALLQAVLKRGLKASCAIIPLMKRDHRDNLEFFLRNVGRLHLLGIDANPNGLFPPVEFPVPRGTPLISPHIKWDHSQVWDVPAAGDFPSGSSGSSTATVYNIDTSPESPDHYLVDHCIDGRVLFPATGYLCLAWKVLARALSQNMEDVPVVFEDVTLHQATILPKSGTVALEVRLLEASRTFEVSQNGNLIVSGKVYQWEDPDPKLFDRGDGPDPADPTDPTATFRLSQGDVYKELRLRGYDYGPYFQGILETNLEGNTGQLLWKDNWVTFLDTMLQMSILGIVQRTLRLPTRITSIHIDPATHRQKVYSLKGQAQVADVVVSSCLNSTAAGGVLISGLHASVAPRRQQEQLAPSLEKFCFTPHVEGGLLAGNAALQEELQLCRGLAQALQTKVAQQGLKMVVPGLDGAQVPREPPQQGLPQLLAAACQLQLNGSLQLELDQLLAQQRPLLHDDPLLSGLLDSPALKACVDTALENLTSPRMYIVLAGDGRLYSRVPALLNTQPMLQLDYTATDRHPQALEAAQAKLQEYGMAQGQWDPADPAPSSLGAADLLVCNCAVAPLRDPATALGNMAAALKEGGFLLLHVLLRGHPLGETVAFLTCPEQRGPQSLLSQDEWEGLFAAASLHLAALKRSFYGSALFLLRRPAPRDSPVFLPVEDTSFRWVDSLKNVMADNSSRPVWLTAVSCATSGVVGMVNCLRREPGGHRIRCILVSNLSSTSPVPKMDPSSSELQKVLQGDLVMNVYRDGSWGAFRHFPLERGLPEEQTEHAFVNVLTRGDLSSIRWVCSPLRHVQPTEPGVYLCTVNYASLNFRDIMLATGKLSPDAIPGKWAARECMLGMEFSGRDASGKRVMGLVPAEGLATSVVLSQDFLWDVPSSWTLEEAASVPVVYTTAYYSLVVRGRVRRGETVLIHSGSGGVGQAAIAIALSLGCRVFTTVGSAEKRAYLQARFPQLSDASFANSRDTSFEQHVLRHTAGKGVDLVLNSLAEEKLQASVRCLAQHGRFLEIGKFDLSNNHQLGMAIFLKNVAFHGILLDSLFDEASSEWREVAGLLQAGIRDGVVQPLKCTTFPKHQVEDAFRYMAQGKHIGKVVVQVSAGPPAPPAPTFCPAHKSYVITGGLGGFGLELAQWLVLRGAQKLVLTSRSGIRTGYQAKQVREWRRQGVQVLVSTSNASSLDGAQSLIAEATQLGPVGGVFNLAMVLRDAMLENQTPEFFQDVSKPKASGTVNLDRVTRAACPELDYFVAFSSVSCGRGNAGQANYGFANSTMERVCEKRRHDGLPGLAVQWGAIGDVGVVLETMGSNDTVIGGTLPQRITSCLEVLDLFLNQPHPVLSSFVLAEKKATAQGDGSTHQDLMKAVAHILGIRDLATVNLDSTLADLGLDSLMGVEVRQMLEREHDLVLSMRDIRQLTLRKLQELSSKASTADAGSPHAQLNLSTLLVNPEGPTLTRLNSVQSSERPLFLVHPIEGSVTVFHSLAAKLSIPTYGLQCTRAAPLDSIQSLAAYYIECIRQVQPEGPYRIAGYSYGACVAFEMCFQLQTQQSPAPTHNSLFLFDGSHTYVLAYTQSYRAKMTRGCEAEAEAEAMCFFLQQFIDMEHDRVLEALLPLGGLEERVAAAVDLIKRSHTGLDRRELSFAARSFYYKLRAAEEYAPRATYHGNVTLLRAKTSSVYGEGLGADYSLSQVCDGKVSVHVIEGDHRTLLEGSGLDSIVSIIHGSLAEPRVSVREG

Computationally mapped potential isoform sequences

There are 4 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A452SJC5A0A452SJC5_URSAMFASN2519
A0A452SJQ2A0A452SJQ2_URSAMFASN2504
A0A452SJR1A0A452SJR1_URSAMFASN2503
A0A452SJH0A0A452SJH0_URSAMFASN2518

Keywords

Genome annotation databases

Similar Proteins

Disclaimer

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