A0A449B5Z3 · A0A449B5Z3_9BACT
- ProteinNH(3)-dependent NAD(+) synthetase
- GenenadE
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids263 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.
Catalytic activity
- deamido-NAD+ + NH4+ + ATP = AMP + diphosphate + NAD+ + H+
Pathway
Cofactor biosynthesis; NAD+ biosynthesis; NAD+ from deamido-NAD+ (ammonia route): step 1/1.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 51 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 126 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain | ||||
Sequence: R | ||||||
Binding site | 146 | ATP (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 151 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 159 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain | ||||
Sequence: K | ||||||
Binding site | 166 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits | ||||
Sequence: D | ||||||
Binding site | 175 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 197 | ATP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 251-252 | deamido-NAD+ (UniProtKB | ChEBI); ligand shared between two neighboring subunits; in other chain | ||||
Sequence: HK |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | glutaminase activity | |
Molecular Function | metal ion binding | |
Molecular Function | NAD+ synthase (glutamine-hydrolyzing) activity | |
Molecular Function | NAD+ synthase activity | |
Biological Process | NAD biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNH(3)-dependent NAD(+) synthetase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Mycoplasmatota > Mycoplasmoidales > Metamycoplasmataceae > Mycoplasmopsis
Accessions
- Primary accessionA0A449B5Z3
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Interaction
Subunit
Homodimer.
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 23-254 | NAD/GMP synthase | ||||
Sequence: LATIERFLKNKVAKAHAQGVVVGISSGIDSSLVFAIAKRVFGAKAIGVVMPVIKMTQSDLNHIKELEIATNSKFMVKDLTKTFESITQELQVSNPLAVANIKPRLRMTTLYAIAQENNSLVLGTDNADEVFVGYFTKFGDGGADLLPISKLTKGEVAYLASLVGVPESIIKKDPSAGLWEGQTDEQELGFSYAQLDFYLNHLNEPKKLKEVLSDKVIAKIEQLHKNSQHKRD |
Sequence similarities
Belongs to the NAD synthetase family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length263
- Mass (Da)29,134
- Last updated2019-05-08 v1
- Checksum274264B1D4BCFD59
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
LR215039 EMBL· GenBank· DDBJ | VEU76021.1 EMBL· GenBank· DDBJ | Genomic DNA |