A0A443WRJ6 · A0A443WRJ6_9ENTR

  • Protein
    Penicillin-binding protein 1B
  • Gene
    mrcB
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    4/5

Function

function

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.

Catalytic activity

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site234Proton donor; for transglycosylase activity
Active site511Acyl-ester intermediate; for transpeptidase activity

GO annotations

AspectTerm
Cellular Componentmembrane
Cellular Componentpeptidoglycan-based cell wall
Molecular Functionpenicillin binding
Molecular Functionpeptidoglycan glycosyltransferase activity
Molecular Functionserine-type D-Ala-D-Ala carboxypeptidase activity
Biological Processcell wall organization
Biological Processpeptidoglycan biosynthetic process
Biological Processproteolysis
Biological Processregulation of cell shape
Biological Processresponse to antibiotic

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Penicillin-binding protein 1B
  • Short names
    PBP-1b
    ; PBP1b
  • Alternative names
    • Murein polymerase

Gene names

    • Name
      mrcB
    • Synonyms
      ponB
    • ORF names
      DN619_00300

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • GEO_33_Up_A
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Klebsiella/Raoultella group > Klebsiella

Accessions

  • Primary accession
    A0A443WRJ6

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane65-87Helical

Keywords

  • Cellular component

Family & Domains

Features

Showing features for region, domain, compositional bias.

Type
IDPosition(s)Description
Region1-61Disordered
Domain1-80Transglycosylase PBP1b N-terminal transmembrane
Domain114-198Bifunctional transglycosylase second
Domain208-380Glycosyl transferase family 51
Domain474-715Penicillin-binding protein transpeptidase
Compositional bias804-835Polar residues
Region804-851Disordered

Sequence similarities

In the C-terminal section; belongs to the transpeptidase family.
In the N-terminal section; belongs to the glycosyltransferase 51 family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    851
  • Mass (Da)
    94,943
  • Last updated
    2019-05-08 v1
  • Checksum
    4DA12E75A503525E
MAGDDREPIGRKGRPSRPAKQKITRRRVREEDYDDDYDDDDYEDEKPMPRKGKGKGGKPPRRKRAWLWLLIKIGVVCAVLIAAYGVYLDQKIRSRIDGKVWDLPAAVYGRMVNLEPDMQISKNEMVRLLNATQYRQVSAMTRPGEYTVQANSIEMIRRPFDFPDSKEGQVRARLTFNGDRLDTIENMDNNRQFGFFRLDPRLITMLQSANGEQRLFVKRNGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFLSSERSYWRKINEAYMALIVDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFGRPVEELSLDQQALLVGMVKGASVYNPWRNPKLALERRNLVLRLLQQQQVIDQELYDMLSARPLGVQPRGGVISPQPAFMQMVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEKAATEGIPALKKQRKLPDLETAMVVVDRFTGEVRAMVGGAEPQFAGYNRAMQARRSIGSLAKPATYLTALSQPNQYRLNTWIADAPISIRLSNGQTWSPQNDDRRYSDSGKVMLVDALTRSMNVPTVNLGMALGLPAVVDTWTKLGAPKNQLNAVPSMLLGALNLTPIEVAQAFQTIASGGNRAPLSALRSVIAEDGTVLYQSYPQAERAVPAQAAYMTLWTMQQVVQRGTGRQLGAKYPGLHLAGKTGTTNNNVDTWFAGIDGGQVTITWVGRDNNQPTKLYGASGAMSIYQRYLANQTPTPLVLTPPEDVVDMGVDGSGNFVCSGGMRSLPVWTTSPDALCRQGEQIQQQQLEQQQANNPFNQSGQQPQQQNQQQQPPKQEKSDGVAGWIKDMFGSN

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias804-835Polar residues

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
QKPB01000001
EMBL· GenBank· DDBJ
RWT49029.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
Help